A Novel Cold Active Esterase from the Marine Psychrotolerant Isolate Pseudoalteromonas Arctica sp. nov.

ISBN-13: 9783833466441 / Angielski / Miękka / 2006 / 138 str.

Rami Al Khudary

Opis

In recent years, growing attention has been devoted to cold adapted microorganisms and their enzymes. A gene library was constructed using the genome of a new aerobic psychrotolerant marine isolate Pseudoalteromonas arctica sp. nov. The gene encoding an esterase was identified by screening the library on tributyrin plates. The recombinant protein was purified (3,3 - fold) in one step using a Ni- column and was subsequently characterised. The enzyme has unique properties since it requires a very low activation energy and retains 50% of its maximal activity at the freezing point of water.

O autorze

Rami Al KhudaryBorn in Tripoli - Lebanon 19.11.1977 Educational background.October 2006: A PHD (Doctor rer. nat.) degree at the Technical University of Hamburg - Harburg (TUHH) "Institute of Technical Microbiology & Biotechnology".June 2002: M.S. in Biology; Lebanese American University - Byblos (LAU).July 1999: Teaching Diploma (for the Intermediate and Secondary classes); American University of Beirut (AUB).July 1998: B.S. in Biology; American University of Beirut (AUB).June 1995: Received the Lebanese Baccalaureate (II) at the Tripoli Evangelical School for Girls & Boys (TES) (Experimental Sciences Section) Projects and esearch.Worked on the screening, cloning, purification and characterization of esterases, lipases, phytases and pullulanases (TUHH)..Joined an expedition to the Azores Islands (Portugal) to collect volcanic water and sediment samples for the isolation and screening of extremophilic microorganisms and enzymes. (TUHH).Biodegradation of Olive oil rich Wastewater under Aerobic & Mesophilic Conditions (LAU). .M.S. Thesis - Isolation and study of an extremely thermostable lipase of an olive oil degrading Thermoactinomyces sp.- (LAU).Whey utilization for the production of Single cell proteins.(LAU)