DNA Profiling and DNA Fingerprinting

ISBN-13: 9783764360184 / Angielski / Miękka / 1999 / 252 str.

Jorg T. Epplen; Thomas Lubjuhn

Opis

This manual presents practical approaches to using DNA fingerprinting and genetic profiling to answer a variety of biological and medical questions. It provides detailed methodology for setting up and performing experiments and evaluating results. Extensive troubleshooting tips, helpful hints, and advice for daily practice are also included. This will be a useful guide for scientists and researchers engaged in genetic identification and relationship analyses.

Spis treści

1. DNA Fingerprinting of Prokaryotic Genomes.- 2. Plant DNA Fingerprinting and Profiling.- 3. DNA Fingerprinting and Profiling in Behavioural Ecology.- 4. DNA Profiling in Veterinary Medicine.- 5. Multilocus DNA Fingerprinting.- 6. Various Levels of (Epi)Genetic Diversities as Demonstrable via Simple Repeated Sequences.- 7. Forensics: Analysis of Short Tandem Repeat Loci by Multiplex PCR and Real Time Fluorescence Detection During Capillary Electrophoresis.- 8. Mitochondrial DNA: Diversity Analysis and Possible Pitfalls.- 9. PCR-based DNA Profiling of Human Y Chromosomes.- 10. The Use of Imperfect Microsatellites for DNA Fingerprinting and Population Genetics.- 11. PCR-SSCP: A Method for Reliably Detecting Single Nucleotide Polymorphisms in Multifactorial Diseases.- 12. Amplified Fragment Length Polymorphisms: A Non-Random PCR-Based Technique for Multilocus Sampling.- 13. Two-Dimensional DNA Fingerprinting.- 14. Statistical Approaches and Methods in Population Genetics Using Microsatellite Data.- 15. Statistical Inference from DNA Evidence.- Guide to Solutions.- Guide to Protocols.- Troubleshooting Guide.