Basil Proteomics

ISBN-13: 9783659235696 / Angielski / Miękka / 2012 / 80 str.

Dr Vikas Sharma;Vikash Singh Jadon

Opis

As a part of the systematic and proteomic approach based characterization of plant material with the advantage of producing valuable informations of functional genomics for a general crop improvement programme, leaves of holy medicinal-aromatic plant Tulsi (Ocimum basilicum L.) were used as a source of proteins and a model eukaryotic system. In this study, we developed a reliable protocol for PEG mediated enrichment of low abundant cellular proteins to increase their detection through 2 DE-SDS PAGE. I6% PEG was found to be optimum for the precipitation of Ocimum protein. Further, proteome proteins in different fractions were analysed electrophoretically including 2- DE technique. These protocols may also will helpful in further studies in basil proteomics and other plants also.

O autorze

Vikas Sharma has done his MSc. and Ph.D in Biotechnology From SBSPGI, Balawala (Dehradun)/ HNB Garhwal Central University, Srinagar, India. He has qualified reputed GATE-BT. Presently he is Asst. Professor, Biotechnology in Arni University,Kathgarh, Himachal Pradash (India). His current area of research is Plant microbe interaction and Biotization.