Angiogenesis Protocols

ISBN-13: 9781627038010 / Angielski / Miękka / 2014 / 358 str.

Stewart Martin; Cliff Murray

Opis

As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology(TM) series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls. Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Recenzje

Aus den Rezensionen zur 2. Auflage: "Dieses Buch, das auch als Onlineversion zu beziehen ist, wendet sich vor allem an Wissenschaftler, deren Forschungsbereich die molekulare Medizin, Kardiologie, Onkologie oder Pharmakologie ist. Die jetzt vorliegende zweite, aktualisierte Ausgabe bietet eine einzigartige Sammlung der verschiedenen Methoden der Zellisolierung und Messung der Angiogenese in vivo und vitro. Detaillierte Laborprotokolle sind genauso enthalten wie Listen mit notwendigen Materialien und Reagenzien und Tipps zur Fehlersuche ..." (in: Geburtshilfe und Frauenheilkunde, 2010, Issue 3, S. 12)

Spis treści

Section I: Review Articles Chapter 1. Angiogenic Signalling Pathways Zubair Ahmed and Roy Bicknell Chapter 2. Imaging Angiogenesis Natalie Charnley, Stephanie Donaldson, and Pat Price Section II: Microscopic Assessment Chapter 3. Assessing Tumor Angiogenesis in Histological Samples Stephen B Fox Chapter 4. Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis Steven Clasper and David G. Jackson Section III: Cell Isolation Techniques Chapter 5. Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture Peter W Hewett Chapter 6. Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines Riccardo E. Nisato and Michael S. Pepper Chapter 7. Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells Adrian T Churchman and Richard CM Siow Chapter 8. Circulating Endothelial Progenitor Cells (CEPC) Michael C. Schmid and Judith A. Varner Section IV: In vitro Techniques Chapter 9. In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis Suzanne A Eccles, William Court, Lisa Patterson and Sharon Sanderson Chapter 10. Tube Formation: an In Vitro Matrigel Angiogenesis Assay M. Lourdes Ponce Chapter 11. Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model David C. West and Mike F. Burbridge Chapter 12. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature Lynn M. Butler, Helen M. McGettrick, and Gerard B. Nash Chapter 13. Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction Eckart Bertelmann Chapter 14. Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing Ira M. Herman and Alice Leung Section V: In vivo Techniques Chapter 15. Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay Andrew V Benest and David O Bates Chapter 16. Quantitative Estimation of Tissue Blood Flow Rate Gillian M Tozer, Vivien E Prise and Vincent J Cunningham Chapter 17. In Vivo Matrigel Migration and Angiogenesis Assay Katherine M. Malinda Chapter 18. The Sponge Implant Model of Angiogenesis Silvia Passos Andrade and Mônica Alves Neves Diniz Ferreira Chapter 19. The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy Axel Sckell and Michael Leunig Chapter 20. The Corneal Pocket Assay Marina Ziche and Lucia Morbidelli Chapter 21. Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature Steven D. Shnyder Chapter 22. The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital Microscopy Axel Sckell and Frank M. Klenke

Blurb

As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity.  Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro.  This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow.  Written in the Methods in Molecular Biology™ series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.

Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.