Microfluidic Diagnostics: Methods and Protocols

ISBN-13: 9781627031332 / Angielski / Twarda / 2013 / 525 str.

Gareth Jenkins; Colin D. Mansfield

Opis

Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microfluidic Diagnostics: Methods in Molecular Biology seeks to aid scientists in the further development and commercialization of microfluidic diagnostic technologies

Spis treści

Section 1: Microfluidic Diagnostics: From the Classroom to the Boardroom

1. Present Technology and Future Trends in Point of Care Microfluidic Diagnostics

Lawrence Kulinsky, Zahra Noroozi, and Marc Madou

2. Teaching Microfluidic Diagnostics Using Jell-O Chips

Cheng Wei T. Yang and Eric T. Lagally

3. Fundamentals of Microfluidics for High School Students with No Prior Knowledge of Fluid Mechanics

Vishal Tandon and Walter Peck

4. Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method

Chenren Shao and Don L. DeVoe

5. Overview of the Microfluidic Diagnostics Commercial Landscape

L. Kim

6. Practical Aspects of the Preparation and Filing of Patent Applications

Fiona Bessoth

7. Introduction to In Vitro Diagnostic Device Regulatory Requirements

Jonathan Day

Section 2. Microfluidic Diagnostics: Fabrication and Manipulation Protocols

8. Microfluidic Device Fabrication by Thermoplastic Hot-Embossing

Shuang Yang and Don L. DeVoe

9. Introduction to Glass Microstructuring Techniques

Radoslaw Mazurczyk, Colin Mansfield, Marcin Lygan

10.Glass Microstructure Capping and Bonding Techniques

Radoslaw Mazurczyk, Colin Mansfield, and Marcin Lygan

11. Rapid Prototyping of PDMS Device using SU-8 Lithography

Gareth Jenkins

12. Microfluidic Interface Technology Based on Stereolithography for Glass-based Lab-on-a-Chips

Song-I Han and Ki-Ho Han

13. Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays

Scott T. Phillips and Nicole K. Thom

14.  Thread Based Devices for Low-Cost Diagnostics

Meital Reches

15. Droplet Based Microfluidics

Sanjiv Sharma, Monphichar Srisa-Art, Steven Scott, Amit Asthana, and Anthony Cass

16. Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET

Monipchar Srisa-Art and Sanjiv Sharma

17. Surface Treatments for Microfluidic Biocompatibility

N.J. Shirtcliffe and P. Roach

18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces

N.J. Shirtcliffe, and P. Roach

Section 3. Microfluidic Diagnostics: Application Protocols

19. The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries

Samantha M. Hattersley, John Greenman, and Stephen J. Haswell

20. Applications of Microfluidics for Molecular Diagnostics

Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale

21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethysiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers

Peng Li

22. Breast Cancer Diagnostics using Microfluidic Multiplexed Immunohistochemistry

Minseok S. Kim, Seyong Kwon, and Je-Kyun Park

23. Charged-couple Device (CCD) Detectors for Lab-on-a Chip (LOC) Optical Analysis

Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck

24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels

Michael P. Cuchiara and Jennifer L. West

25. Purifications of DNA/RNA in a Microfluidic Device

Andy Fan, Samantha Byrnes, and Catherine Klapperich

26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR

Xuefei Leng and Chaoyong James Yang

27. Intergrated Fluidic Circuits (IFCs) for Digital PCR

Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver

28. microFIND^®  Approach to Fluorescent in Situ Hybridization (FISH)

Andrea Zanardi, Emanuele Barborini, and Roberta Carbone

29. An ELISA Lab-on-a-CHOP (ElISA-LOC)

Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov

30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis

Eric Ouellet, Louise Lund, and Eric T. Lagally

31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement

Kerstin Länge, Friederike J. Gruhl, and Michael Rapp

32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins

Junjie Ou, Carolyn L. Ren