Mycobacteria Protocols

ISBN-13: 9781617378263 / Angielski / Miękka / 2011 / 456 str.

Tanya Parish; Amanda Claire Brown

Opis

Methods to study the biology of mycobacteria are still very much in need. This new edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field.

Recenzje

 

Aus den Rezensionen zur 2. Auflage:

"... Fast zehn Jahre nach der ersten Ausgabe ist eine vollständige Überarbeitung erfolgt und zahlreiche Methoden erscheinen erstmalig. ... Eine übersichtliche Gliederung der Kapitel in Einleitung, Material, Methode und Bemerkungen ist hilfreich beim Lesen. In den Bemerkungen stehen viele wichtige Hinweise zur Testdurchführung und auch zur Auswertung der Ergebnisse. ... Das Spektrum der dargestellten Methoden und Arbeitsvorschriften ist sehr breit ... Das vorliegende Methodensammelwerk ist primär für den auf dem Gebiet der Mykobakterien forschenden Molekularbiologen ein wertvolles Handbuch ..."(Dr. A. Reinecke, in: LaboratoriumsMedizin - Journal of Laboratory Medicine, 2009, Vol. 33, Issue 3, S. 176)

Spis treści

Table of Contents Isolation of Mycobacterium ssp. Genomic DNA. John T. Belisle*, Spencer B. Mahaffey and Preston J. Hill. Isolation of Mycobacterial RNA. Tige R. Rustad, David M. Roberts, Reiling P. Liao and David R. Sherman* Lipoglycans of Mycobacterium tuberculosis: isolation, purification and characterization. Libin Shi, Jordi B. Torrelles and Delphi Chatterjee* Analysing lipid metabolism: activation and ß-oxidation of fatty acids.Paul Robert Wheeler* Analysis of lipid biosynthesis and location. Paul Robert Wheeler* Whole genome analysis using microarrays. S. J. Waddell,* J. Hinds and P. D. Butcher Use of DNA microarrays to study global patterns of gene expression. Roberta Provvedi, Giorgio Palù, Riccardo Manganelli* Two-dimensional gel electrophoresis-based proteomics of mycobacteria. Jens Mattow*, Frank Siejak, Kristine Hagens, Julia Kreuzeder, Stefan H.E. Kaufmann, Ulrich E. Schaible Transport assays and permeability in pathogenic mycobacteria. Marie-Antoinette Lanéelle & Mamadou Daffé* Continuous culture of Mycobacteria. Joanna Bacon*, Kim Hatch Measuring Minimum Inhibitory Concentrations in Mycobacteria. Frederick A Sirgel, Ian JF Wiid and David Paul van Helden* Rapid screening of inhibitors of Mycobacterium tuberculosis growth using tetrazolium salts. Anita G. Amin, Shiva K. Angala, Delphi Chatterjee and Dean C. Crick* Electroporation of Mycobacteria. Renan Goude* and Tanya Parish Ins and outs of mycobacterial plasmids. Farahnaz Movahedzadeh* and Wilbert Bitter The use of Temperature Sensitive Plasmids in Mycobacteria. Damien Portevin, Wladimir Malaga and ChristopheGuilhot* Heterologous Expression of Genes in Mycobacteria. James A. Triccas* and Anthony A. Ryan Inducible expression systems for mycobacteria. Christopher M. Sassetti* Assaying promoter activity using LacZ and GFP as reporters. Paul Carroll* and Jade James Construction of unmarked deletion mutants in mycobacteria. Houhui Song, Frank Wolschendorf and Michael Niederweis* Construction of targeted mycobacterial mutants by homologous recombination. Sharon L. Kendall* and Rosangela Frita Phage Transposon Mutagenesis. M. Sloan Siegrist and Eric J. Rubin* Gene Essentiality Testing in Mycobacterium smegmatis using Specialized Transduction. Apoorva Bhatt* and William R. Jacobs Jr. Gene switching and essentiality testing. Amanda Claire Brown* Insertion element IS6110-based Restriction Fragment Length Polymorphism (RFLP) Genotyping of Mycobacterium tuberculosis. Robin M. Warren*, Paul D. van Helden, Nicolaas C. Gey van Pittius Typing M. tuberculosis using VNTR analysis. TJ Brown, VN Nikolayevskyy and FA Drobniewski. Molecular Detection of Drug Resistant Mycobacterium tuberculosis with a scanning-frame oligonucleotide microarray. Dmitriy V. Volokhov, Vladimir E. Chizhikov, Steven Denkin, Ying Zhang* MycoDB: an online database for comparative genomics of the Mycobacteria and related organisms. Roy R. Chaudhuri* Environmental amoeba and mycobacterial pathogenesis. Melanie Harriff and Luiz E. Bermudez*

Blurb

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.  Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field.  Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.