Telomerase Inhibition: Strategies and Protocols

ISBN-13: 9781617377167 / Angielski / Miękka / 2011 / 193 str.

Lucy Andrews; Trygve O. Tollefsbol

Opis

This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition. The volume is aimed at biochemists, molecular biologists, cancer researchers, and geneticists.

Recenzje

From the reviews:

"This book contains 14 chapters by active players in the telomerase field. ... Overall this book will be a useful resource for researchers performing telomerase-related experiments." (Lionel Guittat and Jean-Louis Mergny, ChemMedChem, Issue 3, 2008)

Spis treści

Contents: Preface Contributors 1. Methods of Telomerase Inhibition Lucy G. Andrews and Trygve O. Tollefsbol 2. Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell Lines Kai Kraemer, Susanne Fuessel, and Axel Meye 3. hTERT Knockdown in Human Embryonic Kidney Cells Using Double-Stranded RNA Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol 4. RNA Interference Using a Plasmid Construct Expressing Short Hairpin RNA Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol 5. Retrovirus-mediated RNA Interference: Targeting hTERT Through Stable Expression of Short Hairpin RNA Amanda P. Cunningham, Lucy G. Andrews, and Trygve O. Tollefsbol 6. Telomerase Inhibition and Telomere Targeting in Hematopoietic Cell Lines with Small Non-Nucleosidic Synthetic Compounds (BIBR1532) Hesham El Daly and Uwe M. Martens 7. Uses of Telomerase Peptides in Anti-tumor Immune Therapy He Li, Indzi Katik, and Jun-Ping Liu 8. Establishing Cell-based Reporter Systems for the Analysis of hTERT expression Yi-Yuan Huang, Jing-Wen Shih, and Jing-Jer Lin 9. Telomerase RNA Inhibition Using Antisense Oligonucleotide Against Human Telomerase RNA Linked to a 2', 5'-Oligoadenylate (2-5A) Yasuko Kondo and Seji Kondo 10. Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference Shang Li, Mehdi Nosrati, and Mohammed Kashani-Sabet 11. Evaluation of Tankyrase Inhibition in Whole Cells Tomohazu Ohishi, Takashi Tsuruo, and Hiroyuki Seimiya 12. Inhibition of Telomerase by Targeting MAP Kinase Signaling Dakang Xu, He Li, and Jun-Ping Li 13. Screening of Telomerase Inhibitors Elke Kleideiter, Kamilla Piotrowska, and Ulrich Klotz 14. Telomerase Inhibition Combined with Other Chemotherapeutic Reagents to Enhance Anti-Cancer Effect Tetsuzo Tauchi, Junko H. Ohyashiki,and Kazuma Ohyashiki

Blurb

Due in part to the selective nature of telomerase inhibition as an anticancer approach, the field has expanded considerably in the past decade. The recent advances in methods of telomerase inhibition encompass many different areas of research including molecular biology, cell biology, biochemistry, oncology and gerontology. Telomerase Inhibition provides methods and protocols for those researchers. The techniques described in this book should provide the researcher with a diverse and comprehensive set of tools with which to study telomerase inhibition. Leaders in the field provide recently developed methods that have widespread application such as targeting the telomerase holoenzyme, its RNA template and other elements associated with telomerase activity. Additional methods involving the screening of telomerase inhibitors and telomerase inhibition combined with other chemotherapeutic agents are presented. This text, on the cutting edge of the field, will provide investigators with the most recent methods applied to the expanding field of telomerase inhibition.