Cell Migration: Developmental Methods and Protocols

ISBN-13: 9781617375323 / Angielski / Miękka / 2010 / 364 str.

Jun-Lin Guan

Opis

A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls."

Spis treści

Part I. Introduction Cell Migration: An Overview Donna J. Webb, Huaye Zhang, and Alan F. Horwitz Part II. Basic Cell Migration and Related Assays Boyden Chamber Assay Hong-Chen Chen Wound-Healing Assay Luis G. Rodriguez, Xiaoyang Wu, and Jun-Lin Guan Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy Claire M. Wells and Anne J. Ridley Cell-Adhesion Assays Dennis F. Kucik and Chuanyue Wu Cell-Spreading Assays Allison L. Berrier and Susan E. LaFlamme Cell-Scatter Assay Hong-Chen Chen Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices Edna Cukierman Part III. Analysis of Cell Migration in Specialized Cell Systems Tumor Cell Invasion Assays Leslie M. Shaw Analysis of Endothelial Cell Migration Under Flow Song Li Angiogenesis Assays in the Chick CAM Chris Storgard, David Mikolon, and Dwayne G. Stupack Investigations of Neuronal Migration in the Central Nervous System Michael E. Ward and Yi Rao Part IV. Analysis of Cell Migration in Model Organisms Analysis of Cell Migration in Caenorhabditis elegans M. Afaq Shakir and Erik A. Lundquist Analysis of Cell Migration Using Drosophila as a Model System Jocelyn A. McDonald and Denise J. Montell Imaging Cell Movement During Dorsal Closure in Drosophila Embryos William Wood and Antonio Jacinto Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In Vivo Diane S. Sepich and Lilianna Solnica-Krezel The Xenopus Embryo as a Model System for Studies of Cell Migration Douglas W. DeSimone, Lance Davidson, Mungo Marsden, and Dominique Alfandari Neural Crest Migration Methods in the Chicken Embryo Maria Elena de Bellard and Marianne Bronner-Fraser In Vivo and In Vitro Models of Mammalian Angiogenesis Mien V. Hoang and Donald R. Senger Part V. Biochemical and Novel Approaches in Cell Migration Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, and Michael D. Schaller Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane Patches Frauke Drees, Amy Reilein, and W. James Nelson Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells Christoph Ballestrem and Benjamin Geiger Use of Multiphoton Imaging for Studying Cell Migration in the Mouse Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, and Alexander Yu. Nikitin Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, and Jun-Lin Guan Index

Blurb

Central to the better understanding of both molecular mechanisms and disease, cell migration plays an essential role in a variety of biological processes and is now the subject of intense study using an array of powerful new technologies. In Cell Migration: Developmental Methods and Protocols, researchers describe in step-by-step detail their most successful techniques for studying the macromolecular machinery of cell movement. These readily reproducible protocols include a wide range of novel and state-of-the-art methodologies, as well as many classic methods, for use in cultured cells, different model organisms, and specialized cells in both normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The authors also offer several novel approaches to the study of cell migration, as well as extensive coverage of cell migration studies in developmental and disease models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Cell Migration: Developmental Methods and Protocols offers researchers easy access to many readily reproducible techniques for the optimally productive investigation of cell migration in today's interdisciplinary experimental environment.