Complement Methods and Protocols

ISBN-13: 9781617371165 / Angielski / Miękka / 2010 / 259 str.

B. Paul Morgan

Opis

The complement system, first described more than a century ago, was for many years the ugly duckling of the immunology world, but no more. Complement in recent years has blossomed into a fascinating and fast moving field of immediate relevance to clinical scientists in fields as diverse as transplantation biology, virology, and inflammation. Despite its emergence from the shadows, complement retains an unwarranted reputation for being "difficult." This impression derives in large part from the superficially complicated nomenclature, a relic of the long and tortuous process of unraveling the system, of naming components in order of discovery rather than in a syst- atic manner. Once the barrier of nomenclature has been surmounted, then the true simplicity of the system becomes apparent. Complement comprises an activation system and a cytolytic system. The former has diverged to focus on complement to distinct targets-bacteria, - mune complexes, and others-so that texts now describe three activation pa- ways, closely related to one another, but each with some unique features. The cytolytic pathway is the same regardless of the activation process and kills cells by creating pores in the membrane. Complement plays an important role in killing bacteria and is essential for the proper handling of immune complexes. Problems occur when complement is activated in an inappropriate manner-the potent inflammation-inducing products of the cascade then cause unwanted tissue damage and destruction.

Recenzje

recommended for inclusion in biomedical research libraries, both corporate and academic." - E-Streams

Spis treści

The Complement System: An Overview, B. Paul Morgan. Purification of Complement Components, Regulators, and Receptors by Classical Methods, Carmen W. van den Berg. Immunoaffinity Methods for Purification of Complement Components and Regulators, B. Paul Morgan. Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates, B. Paul Morgan. Measurement of Complement Lysis of Nucleated Cells, O. Brad Spiller. Functional Assays for Complement Regulators, Claire L. Harris. Immunochemical Measurement of Complement Components and Activation Products, Reinhard Würzner. Complement Deposition in Tissues, Antti Väkevä and Seppo Meri. Complement Regulators and Receptors in Tissues, Juha Hakulinen and Seppo Meri. Measurement of C3 Fragment Deposition on Cells, O. Brad Spiller. Screening for Complement Deficiency, Ann Orren. C1-Inhibitor: Antigenic and Functional Analysis, C. Erik Hack. Autoantibodies to Complement Components, Kevin A. Davies and Peter Norsworthy. Allotyping of Complement Components, Reinhard Würzner. Complement and Immune Complexes, Julian T. Nash and Kevin A. Davies. Knocking Out Complement Genes, Anne E. Bygrave and Marina Botto. Inherited Complement Deficiencies in Animals, Stuart Linton. Appendices. Suppliers. Sources of C Components and Anti-C Antibodies. cDNA Accession Numbers for C Components. Regulators, and Receptors. Index.

Blurb

Although the complement system-a vital part of the body's defense against bacteria-was discovered more than a century ago, its study has undergone a renaissance with the identification of its regulatory molecules and the realization that these molecules can be used therapeutically. In Complement Methods and Protocols, B. Paul Morgan and a team of expert laboratorians present a comprehensive set of readily reproducible methods to study this critical system. These cutting-edge techniques are suitable both for the basic scientist interested in understanding complement's mechanisms of activation and for the clinical scientist wishing to quantify its activation, and range from the purification of its components to generating complement-deficient mice by gene deletion. Additional techniques presented include procedures for the analysis of complement function, for the study of its regulators, for detection of its activation in vivo, and for the identification of its autoantibodies.
Comprehensive and cutting-edge, Complement Methods and Protocols offers today's basic and clinical investigators powerful tools for the analysis of the role of complement in human pathophysiology and disease, as well as its therapeutic regulation.