Clostridium Difficile: Methods and Protocols

ISBN-13: 9781603273640 / Angielski / Twarda / 2010 / 241 str.

Peter Mullany; Adam P. Roberts

Opis

Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.

Recenzje

From the reviews:

"This book describes a variety of research methods used to investigate the pathogenic properties of Clostridium difficile, a major nosocomial pathogen that causes a severe diarrhea after prolonged antibiotic treatment. ... This book would be useful to scientists working with bacteria such as C. difficile that cause toxin-mediated diseases in the colon of their host. ... This is a technical book that provides essential methods and techniques to study devastating diseases due to Clostridium difficile." (Rebecca T. Horvat, Doody's Review Service, October, 2010)

Spis treści

Part I: Introduction to C. difficile and the Disease It Causes 1. Clostridium difficile: No Longer an Enigmatic Pathogen? Adam P. Roberts and Peter Mullany 2. Clostridium difficile and the Disease It Causes Torbjörn Norén Part II: Isolation and Culture Techniques 3. Clostridium difficile Isolation and Culture Techniques Mike Wren Part III: Methods for Typing and Epidemiological Studies 4. Molecular Typing Methods for C. difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping Sandra Janezic and Maja Rupnik 5. Clostridium difficile Toxinotyping Maja Rupnik 6. Multilocus Sequence Typing for Clostridium difficile Ludovic Lemée and Jean-Louis Pons Part IV: Biochemistry of the Organism 7. Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes Ana Antunes and Bruno Dupuy 8. Dissecting the Cell Surface Robert Fagan and Neil Fairweather 9. Human Intestinal Epithelial Response(s) to Clostridium difficile Nazila V. Jafari, Elaine Allan, and Mona Bajaj-Elliott Part V: Genomics 10. Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays Richard Stabler, Lisa Dawson, and Brendan Wren Part VI: Development of Systems for Genetic Analysis of the Organism 11. ClosTron-Targeted Mutagenesis John T. Heap, Stephen T. Cartman, Sarah A. Kuehne, Clare Cooksley, and Nigel P. Minton 12. Methods for Gene Cloning and Targeted Mutagenesis Glen P. Carter, Dena Lyras, Rachael Poon, Pauline M. Howarth, and Julian I. Rood 13. Transposon Mutagenesis in C. difficile Haitham A. Hussain, Adam P. Roberts, Rachael Whalan, and Peter Mullany Part VII: Animal Models ofDisease 14. Refinement of the Hamster Model of Clostridium difficile Disease Gillian Douce and David Gouding 15. Methods for Working with the Mouse Model Anne Collignon