Therapeutic Applications of RNAi: Methods and Protocols

ISBN-13: 9781603272940 / Angielski / Twarda / 2009 / 148 str.

Cristina M. Rondinone; John Reidhaar-Olson

Opis

The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular BiologyT series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges

Recenzje

From the reviews: "This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized in 10 chapters dealing with the ... task of delivering RNA interference molecules (RNAi) to live organisms to achieve therapeutic effects. ... The book is written mainly for scientists, clinical researchers, and all investigators interested in the field of biotherapeutic research." (Ruben Mestril, Doody's Review Service, January, 2010)

Spis treści

1. Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors Zdravka Medarova, Mohanraja Kumar, Shu-wing Ng, and Anna Moore 2. Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA Delivery Muriel Golzio, Laurent Mazzolini, Aurélie Paganin-Gioanni, and Justin Teissié 3. Liposomal siRNA for Ovarian Cancer Lingegowda S. Mangala, Hee Dong Han, Gabriel Lopez-Berestein, and Anil K. Sood 4. Therapeutic Applications of RNAi for Silencing Virus Replication Ralph A. Tripp and Stephen Mark Tompkins 5. RNAi in the Malaria Vector, Anopheles gambiae Flaminia Catteruccia and Elena A. Levashina 6. RNAi Using a Chitosan/siRNA Nanoparticle System: in vitro and in vivo Applications Morten Østergaard Andersen, Kenneth Alan Howard, and Jørgen Kjems 7. Lentiviral and Adeno-Associated Vector-Based Therapy for Motor Neuron Disease through RNAi Chris Towne and Patrick Aebischer 8. Studying Autoimmunity by in vivo RNA Interference Stephan Kissler 9. Transcriptional Gene Silencing Using Small RNAs Daniel H. Kim and John J. Rossi 10. Alternative Splicing as a Therapeutic Target for Human Diseases Kenneth J. Dery, Veronica Gusti, Shikha Gaur, John E. Shively, Yun Yen, and Rajesh K. Gaur

Blurb

The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.