Mouse Cell Culture: Methods and Protocols

ISBN-13: 9781588297723 / Angielski / Twarda / 2010 / 257 str.

Ward, Andrew

Opis

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

Recenzje

From the reviews:

"This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. ... This book is recommended for researchers and students who are interested in studying the culture of cells from animal models." (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)

Spis treści

1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester 2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures Alastair S. Garfield 3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem Cells Janet Smith and Deborah Merrick 4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA Interference Jamie A. Davies 5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation Pierre-Marie del Moral and David Warburton 6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh 7. Isolation and Culture of Embryonic Pancreas and Liver Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh 8. Isolation and Culture of Mouse Satellite Cells Antonio Musarò and Laura Barberi 9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton 10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes Reto Caldelari and Eliane J. Müller 11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial Cells Matthew J. Smalley 12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes Magalie A. Ravier and Guy A. Rutter 13. Isolation and Culture of Adult Mouse Hepatocytes Wan-ChunLi, Kate L. Ralphs, and David Tosh 14. Isolation and Culture of Mouse Intestinal Cells F. Charles Campbell 15. Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward 16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry Carla Sciarretta and Liliana Minichiello 17. Cell Culture of Primary Cerebellar Granule Cells Dana Krämer and Liliana Minichiello 18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain Henrik Ahlenius and Zaal Kokaia

Blurb

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.