Chromosome Architecture: Methods and Protocols

ISBN-13: 9781493981007 / Angielski / Miękka / 2018 / 290 str.

Mark C. Leake

Spis treści

New Advances in Chromosome Architecture.- Single Molecule Narrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose Signal Transduction Of Live Yeast Cells.- Single-Molecule Imaging to Characterise the Transport Mechanism of the Nuclear Pore Complex.- Using Fluorescence Recovery after Photobleaching (FRAP) To Study Dynamics Of The Structural Maintenance Of Chromosome (SMC) Complex In Vivo.- Imaging DNA Structure by Atomic Force Microscopy.- Investigating Bacterial Chromosome Architecture Using Epifluorescence Microscopy.- Transverse Magnetic Tweezers Allowing Coincident Epi-Fluorescence Microscopy On Horizontally Extended DNA.- Probing Chromosome Dynamics in Bacillus Subtilis.- Systems Biology Approaches for Understanding Genome Architecture.- In Vivo And In Situ Replication Labeling Methods For Super-Resolution Structured Illumination Microscopy Of Chromosome Territories And Chromatin Domains.- DNA-Protein Interactions Studied Directly Using Single Molecule Fluorescence Imaging Of Quantum Dot Tagged Proteins Moving On DNA Tightropes.- Escherichia Coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis.- Bacterial Chromosome Dynamics by Locus Tracking In Fluorescence Microscopy.- Biophysical Characterization of Chromatin Remodelling Protein CHD4.- Atomistic Molecular Dynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup, Performance and Analysis.- Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells.- Studying the Dynamics of Chromatin Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy.- Intra Nuclear Single Particle Tracking (I-SPT) To Reveal the Functional Architecture Of Chromosomes.- Visualizing Bacillus Subtilis during Vegetative Growth and Spore Formation.