Multiplex Biomarker Techniques: Methods and Applications

ISBN-13: 9781493967292 / Angielski / Twarda / 2016 / 317 str.

Paul C. Guest

Opis

This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Multiplex Biomarker Techniques: Methods and Applications serves as an ideal resource for a wide variety of researchers interested in these vital multiplex techniques.

Spis treści

Part I: Reviews

 

1. Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development

            Hassan Rahmoune and Paul C. Guest

 

2. The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients

            Johann Steiner, Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de-Souza

 

3. Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research

            Susan E. Ozanne, Hassan Rahmoune, Daniel Martins-de Souza, and Paul C. Guest

 

4. LC-MS^E, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics

            Gustavo Henrique Martins Ferreira Souza, Paul C. Guest, and Daniel Martins-de-Souza

 

5. Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies

            James R. Mansfield

 

Part II: Statistical Considerations

 

6. Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations

            Emanuel Schwarz

 

7. Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective

            Junfang Chen and Emanuel Schwarz

 

Part III: Protocols

 

8. Multiplex Analyses Using Real-Time Quantitative PCR

            Steve F.C. Hawkins and Paul C. Guest

 

9. Multiplex Analysis Using cDNA Transcriptomic Profiling

            Steve F.C. Hawkins and Paul C. Guest

 

10. Multiplex Single Nucleotide Polymorphism Analyses

            Steve F.C. Hawkins and Paul C. Guest

 

11. Pulsed SILAC as a Proteomic Approach for miRNA Targets Identification in Cell Culture

            Daniella E. Duque-Guimarães, Juliana de Almeida-Faria, Thomas Prates Ong, and Susan E. Ozanne

 

12. Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies

            Paul C. Guest and Hassan Rahmoune

 

13. Multiplex Immunoassay Profiling

            Laurie Stephen

 

14. Multiplex Sequential Immunopreciptation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets

            Paul C. Guest

 

15. Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets

            Paul C. Guest

 

16. Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders

            Sheila Garcia, Paulo A. Baldasso, Paul C. Guest, and Daniel Martins-de-Souza

 

17. Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Three Distinct Proteomes

            Adriano Aquino, Paul C. Guest, and Daniel Martins-de Souza

 

18. Selective Reaction Monitoring for Quantitation of Cellular Proteins

            Vitor M. Faça

 

19. Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry

            Giuseppina Maccarrone, Juan Jose Bonfiglio, Susana Silberstein, Christoph W. Turck, and Daniel Martins-de-Souza

 

20. Using ¹⁵N-Metabolic Labeling for Quantitative Proteomic Analyses

            Maccarrone Giuseppina, Alon Chen, and Michaela D. Filiou

 

21. Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS

            Sarah Meadows

 

22. UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life

            Shima Assar, Inez Schoenmakers, Albert Koulman, Ann Prentice, and Kerry S. Jones

 

23. iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification

            Erika Velásquez Núñez, Gilberto Barbosa Domont, and Fábio César Sousa Nogueira

 

24. ¹H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples

            João G.M. Pontes, Antonio J.M. Brasil, Guilherme C.F. Cruz, Rafael N. de Souza, and Ljubica Tasic

 

25. Lab-on-a-Chip Multiplex Assays

            Harald Peter, Julia Wienke, and Frank F. Bier

 

26. Multiplex Smartphone Diagnostics

            Juan L. Martinez-Hurtado, Ali K. Yetisen, and Seok H. Yun

 

27. Development of a User-Friendly App for Assisting Anticoagulation Treatment

            Johannes Vegt

 

Part IV: Future Directions

 

28. Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine

            Paul C. Guest

Blurb

This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. <