Natural Products Isolation: Methods and Protocols

ISBN-13: 9781493956616 / Angielski / Miękka / 2016 / 552 str.

Satyajit D. Sarker; Lutfun Nahar

Opis

In its 3rd edition, this Methods in Molecular Biology(TM) volume reviews the latest methods for natural products isolation with a combination of new chapters and revised and expanded classic methods. Includes protocols, and tips on troubleshooting and pitfalls.

Spis treści

1. An Introduction to Natural Products Isolation

            Satyajit D. Sarker and Lutfun Nahar

 

2. Initial and Bulk Extraction of Natural Products Isolation

            Véronique Seidel

 

3. Supercritical Fluid Extraction in Natural Products Analyses

            Lutfun Nahar and Satyajit D. Sarker

 

4. Accelerated Solvent Extraction (ASE^®) for Natural Products Isolation

            Mohammad A. Mottaleb and Satyajit D. Sarker

 

5. Microwave-Assisted Extraction in Natural Products Isolation

            Abbas Delazar, Lutfun Nahar, Sanaz Hamedeyazdan, and Satyajit D. Sarker

 

6. An Introduction to Planar Chromatography and Its Application to Natural Products Isolation

            Simon Gibbons

 

7. Isolation of Natural Products by Low-Pressure Column Chromatography

            Raymond G. Reid and Satyajit D. Sarker

 

8. Isolation of Natural Products by Ion-Exchange Methods

            Klaus Dragull and John J. Beck

 

9. Separation of Natural Products by Countercurrent Chromatography

            James B. McAlpine, J. Brent Friesen, and Guido F. Pauli

 

10. Isolation of Natural Products by Preparative High Performance Liquid Chromatography (Prep-HPLC)

            Zahid Latif and Satyajit D. Sarker

 

11. Isolation of Natural Products by Preparative Gas Chromatography

            Temel Özek and Fatih Demirci

 

12. Hyphenated Techniques and Their Applications in Natural Products Analysis

            Satyajit D. Sarker and Lutfun Nahar

 

13. Extraction of Plant Secondary Metabolites

            William P. Jones and A. Douglas Kinghorn

 

14. Isolation of Marine Natural Products

            Wael E. Houssen and Marcel Jaspars

 

15. Isolation of Microbial Natural Products

            Olov Sterner

 

16. Extraction and Isolation of Saponins

            Runner R.T. Majinda

 

17. Extraction and Isolation of Phenolic Compounds

            Celestino Santos-Buelga, Susana Gonzalez-Manzano, Montserrat Dueñas, and Ana M. Gonzalez-Paramas

 

18. Scaling-Up of Natural Products Isolation

            Yash Kumarasamy

 

19. Follow-Up of Natural Products Isolation

            Richard J.P. Cannell, Satyajit D. Sarker, and Lutfun Nahar

 

20. Natural Products Isolation in Modern Drug Discovery Programs

            Alexander I. Gray, John O. Igoli, and RuAngelie Edrada-Ebel

Blurb

With significant developments in the areas of chromatography and spectroscopy as well as the unique inherent chemical diversity of natural products, vital in drug research, natural products research has gained new momentum.  Fully updating and adding to the previous two editions, Natural Products Isolation, Third Edition documents the latest methods and technologies for natural products isolation with a combination of all new chapters and revised and expanded classic methods.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Natural Products Isolation, Third Edition provides the substantial background information needed by budding natural product researchers as well offering an invaluable reference guide to available methodologies and techniques for the more experienced researchers.