C. Elegans: Methods and Applications

ISBN-13: 9781493928415 / Angielski / Twarda / 2015 / 252 str.

David Biron; Gal Haspel

Opis

The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, C. elegans: Methods and Applications, Second Edition aims to ensure successful results in the further study of this dynamic field.

Spis treści

1. Library Construction for Mutation Identification by Whole-Genome Sequencing

Harold E. Smith

 

2. Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes

Eric S. Haag and Cristel G. Thomas

 

3. Genetic Methods for Cellular Manipulations in C. elegans

Menachem Katz

 

4. A Fusion PCR Method for Expressing Genetic Tools in C. elegans 

Yifat Eliezer and Alon Zaslaver

 

5. Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI)

Christian Frøkjær-Jensen

 6. Creating Genome Modifications in C. elegans using the CRISPR/Cas9 SystemJohn A. Calarco and Ari E. Friedland

 

7. Observing and Quantifying Fluorescent Reporters

Michael Hendricks

 

8. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior

Caspar Glock, Jatin Nagpal, Alexander Gottschalk

 

9. Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans

Nicholas F Trojanowski and Christopher Fang-Yen

 

10. High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans

Laura Manning and Janet Richmond

 

11. Electron Tomography Methods for C. elegans

David H. Hall and William J. Rice

 

12. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans

Ross C. Lagoy and Dirk R. Albrecht

 

13. Tracking Single C. elegans using a USB Microscope on a Motorized Stage

Eviatar I. Yemini and André E.X. Brown

 

14. An Imaging System for C. elegans Behavior

Matthew A. Churgin and Christopher Fang-Yen

 

15. A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae

Maria C. Ow and Sarah E. Hall

 

16. Sampling and Isolation of C. elegans from the Natural Habitat

Nausicaa Poullet  and Christian Braendle

 

17. A Primer on Prototyping

Dylan Lynch and David Biron

 

18. A Primer on Quantitative Modeling

Iulia Neagu and Erel Levine