Gene Therapy of Solid Cancers: Methods and Protocols

ISBN-13: 9781493927265 / Angielski / Twarda / 2015 / 387 str.

Wolfgang Walther; Ulrike Stein

Opis

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy. Gene Therapy of Solid Cancers: Methods and Protocols guides readers through protocols on gene therapeutic strategies in combination with helpful technical notes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, Gene Therapy of Solid Cancers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

Part I: Immunogene Therapy

1. Aptamer Targeting the ERBB2 Receptor Tyrosine Kinase for Applications in Tumor Therapy

Georg Mahlknecht, Michael Sela, and Yosef Yarden

 

2. Gene Gun Her2/neu DNA Vaccination: Evaluation Of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model

Tam Nguyen-Hoai, Antonio Pezzutto, and Jörg Westermann

 

3. MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine For Cancer Therapy

Manuel Schmidt, Barbara Volz, Patrick Großmann, Kerstin Heinrich, and Burghardt Wittig

 

Part II: Suicide Gene Therapy

4. Evaluation of Bystander Cell Killing Effects in Suicide Gene Therapy of Cancer: Engineered Thymidylate Kinase (TMPK)/AZT Enzyme-Prodrug Axis

Takeya Sato, Anton Neschadim, Ryo Nakagawa, Teruyuki Yanagisawa, and Jeffrey A. Medin

 

5. Oncoleaking: Use of the Pore-forming Clostridium perfringens Enterotoxin (CPE) for Suicide Gene Therapy

Jessica Pahle, Jutta Aumann, Dennis Kobelt, and Wolfgang Walther

 

6. iCaspase 9 Suicide Gene System

Xiaoou Zhou, Antonio Di Stasi, and Malcolm K. Brenner

 

Part III: Gene Replacement Therapies

7. p53-encoding pDNA Purification by Affinity-Chromatography for Cancer Therapy

Ângela Sousa, João A. Queiroz, and Fani Sousa

 

8. A qRT-PCR Method for Determining the Biodistribution Profile of a miR-34a Mimic

Kevin Kelnar and Andreas G. Bader

 

Part IV: Gene Suppression and Signaling Modulation Therapy

9. Design and Selection of Antisense Oligonucleotides Targeting Transforming Growth Factor Beta (TGF-b) Isoform mRNAs for the Treatment of Solid Tumors

Frank Jaschinski, Hanna Korhonen, and Michel Janicot

 

10. RNA Interference for Antimetastatic Therapy

Mathias Dahlmann  and Ulrike Stein 

 

11. STAT3 Decoy ODN Therapy for Cancer

Xiaoxia Sun and Jian Zhang

 

Part V: Oncolytic Virotherapy and Bacterial Vectors for Cancer Treatment

12. Oncolytic Viral Therapy using Reovirus

Chandini Thirukkumaran and Don G. Morris

 

13. Use of GLV-1h68 for Vaccinia Virotherapy and Monitoring

Alexa Frentzen, Ulrike Geissinger, Desislava Tsoneva, and Jochen Stritzker

 

14. Back To The Future: Are Tumor-Targeting Bacteria The Next Generation Cancer Therapy?

Robert M. Hoffman

 

 

Part IV: Clinical Application of Cancer Gene Therapy

15. Ethics of Cancer Gene Transfer Clinical Research

Jonathan Kimmelman

 

16. Planning an Academic Clinical Trial

Kim M. Champion and Gemma R. Jones

 17. Production of Plasmid DNA as Pharmaceutical

Marco Schmeer and Martin Schleef

 

18. Minicircle - Next Generation DNA Vectors for Vaccination

Schleef, M., Schirmbeck, R., Michael Reiser², M.-L. Michel, and Schmeer, M.

 

VII: Protocols of Clinical Cancer Gene Therapy

19. A Phase 2, Open-Label, Randomized Study of Pexa-Vec (JX-594) Administered by Intratumoral Injection in Patients with Unresectable Primary Hepatocellular Carcinoma

Caroline J. Breitbach, Anne Moon, James Burke, Tae-Ho Hwang, and David H. Kirn

 20. Antiangiogenic Metargidin Peptide (AMEP) Gene Therapy in Disseminated Melanoma

Iben Spanggaard and Julie Gehl

 21. Clinical Evaluation of ErbB-targeted CAR T-cells, Following Intra-Cavity Delivery in Patients with ErbB-Expressing Solid Tumors

Sophie Papa, May van Schalkwyk, and John Maher