Yeast Genetics: Methods and Protocols

ISBN-13: 9781493913626 / Angielski / Twarda / 2014 / 378 str.

Jeffrey S. Smith; Daniel J. Burke

Opis

Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.

Spis treści

1. Yeast Transformation by LiAc/SS Carrier DNA/PEG Method

            R. Daniel Gietz

 

2. Tetrad, Random Spore and Molecular Analysis of Meiotic Segregation and Recombination

            Michael Lichten

 

3. PCR Mutagenesis and Gap Repair in Yeast

            Mark Weir and Jill B. Keeney

 

4. PCR-Mediated Epitope Tagging of Genes in Yeast

            Radhika Mathur and Peter Kaiser

 

5. Manipulating the Yeast Genome: Deletion, Mutation and Tagging by PCR

            Jennifer M. Gardner and Sue L. Jaspersen

 

6. Preparation of Yeast Cells for Live Cell Imaging and Indirect Immunofluorescence

            Lucy F. Pemberton

 

7. Single Yeast Cell Imaging

            Heimo Wolinski and Sepp D. Kohlwein

 

8. Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells

            Anjali Bisaria, Pascal Hersen, and Megan N. McClean

 

9. Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations   

            Brian Haarer and David C. Amberg

 

10. Synthetic Genetic Array Analysis of Global Mapping of Genetic Networks in Yeast

            Elena Kuzmin, Sara Sharifpoor, Anastasia Baryshnikova, Michael Costanzo, Chad L. Myers, Brenda J. Andrews, and Charles Boone

 

11. Chemical Genetic and Chemogenomic Analysis in Yeast

            Namal V. C. Coorey, Liam D.P. Sampson, Jacqueline M. Barber, and David S. Bellows

 

12. Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays

            John Rodgers, Jingyu Guo, and John L. Hartman, IV

 

13. Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast

            Badri Nath Singh and Michael Hampsey

 

14. Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast

            Maria D. Mayán and Luis Aragón

 

15. Global Analysis of Transcription Factor Binding Sites in Yeast Using ChIP-Seq

            Philippe Lefrançois, Jennifer E.G. Gallagher, and Michael Snyder

 

16. High-Density Tiling Microarrays Analysis of the Full Transcriptional Activity of Yeast

            David Lior, Sandra Clauder-Münster, and Lars M. Steinmetz

 

17. Analysis of Silencing in Saccharomyces cerevisiae

            Andrew Miller and Ann L. Kirchmaier

 

18. A User’s Guide to the Ribosomal DANN in Saccharomysces cerevisiae

            Joseph M. Johnson, Jeffrey S. Smith, and David A. Schneider

 

19. Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication       

            Sandra K. Villwock and Oscar M. Aparicio

 

20. Replicative Life Span Alaysis in Budding Yeast

            George L. Sutphin, Joe R. Delaney, and Matt Kaeberlein

 

21. Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast

            Vincent R. Richard, Simon D. Bourque, and Vladimir I. Titorenko