Amino Acids

ISBN-13: 9781489941367 / Angielski / Miękka / 2013 / 281 str.

Alan A. Boulton; Glen B. Baker; James D. Wood

Opis

Techniques in the neurosciences are evolving rapidly. There are currently very few volumes dedicated to the methodology - ployed by neurosclentlsts, and those that are available often seem either out of date or limited m scope. This series is about the methods most widely used by modern-day neurosclentists and 1s wrltten by their colleagues who are practicing experts. Volume 1 will be useful to all neurosclentists since It concerns those procedures used routinely across the widest range of subdisciplines. Collecting these general techniques together m a single volume strikes us not only as a service, but will no doubt prove of exceptIona utilitarian value as well. Volumes 2 and 3 - scribe all current procedures for the analyses of amines and their metabolltes and of ammo aads, respectively. These collections will clearly be of value to all neuroscientlsts working m or contemplating research m these fields. Similar reasons exist for Volume 4 on receptor binding techniques since experimental - tails are provided for all types of llgand-receptor bmdmg, including chapters on general prmciples, drug discovery and - velopment, and a most useful appendix on computer programs for Scatchard, nonlinear, and competitive displacement analyses. Volume 5 provides procedures for the assessment of enzymes - volved m biogenic amme synthesis and catabolism. Volumes in the NELJROMETHODS series will be useful to neurochemists, -pharmacologists, -physiologists, -anatomlsts, psychopharmacologists, psychlatnsts, neurologists, and chemists (organic, analytical, pharmaceutical, medicinal), in fact, everyone involved m the neurosclences, both basic and clinical.

Spis treści

Automated Amino Acid Analysis.- Gas Chromatographic Analysis of Amino Acids.- Gas Chromatography—Mass Fragmentography of Amino Acids.- Double-Isotope Dansyl Microassay for Cerebral Amino Acids.- Liquid Chromatographic Determination of Amino Acids After Precolumn Fluorescence Derivatization.- Radioreceptor Assays for Amino Acids and Related Compounds.- Immunocytochemical Techniques.- In vitro Autoradiographic Localization of Amino Acid Receptors and Uptake Sites.- Determination of Transmitter Amino Acid Turnover.- Uptake and Release of Amino Acid Neurotransmitters.