Euglena: An Experimental Organism for Biochemical and Biophysical Studies

ISBN-13: 9781468460599 / Angielski / Miękka / 2012 / 204 str.

Jerome J. Wolken; Jerome J. Wolken

Opis

attempted to cover metabolic matters already reviewed in Volumes I and II of The Biochemistry and Physiology of Protozoa (eds. Lwoff, and Lwoff and Hutner). To those interested in the broader aspects of photo synthesis and photoreception, reference is made to Photosynthesis and Related Processes, Volume I, and parts I and 2 of Volume II, by E. Rabinowitch; the Brookhaven Symposium on The Photochemical Ap paratus: Its Structure and Function (1959); the New York Academy of Sciences conference on Photoreception (1958), and to the many recent symposia, reviews, and current journal literature. This book is primarily concerned with the use of Euglena in study ing photoreception; it is also hoped that biologists, biochemists, and biophysicists will find in Euglena a remarkably versatile research tool for attacking some of their problems. I would like to thank Drs. S. H. Hutner and L. Provasoli of the Haskins Laboratories for introducing me to Euglena as an exceptional experimental "animal," as well as for many stimulating discussions and continued enthusiasm. I am indebted to Drs. G. E. Palade and K. R.

Spis treści

1 Introduction.- Characteristics of Euglena.- 2 Experimental Methods.- Electron Microscopy.- Spectroscopy.- Microspectrophotometry.- 3 Growth, Metabolism, and Structure.- Structure.- 4 Pigments.- Chlorophylls.- Carotenoids.- Pigment Analysis of Euglena.- Microspectrophotometry.- Cytochromes.- 5 Chloroplast Structure.- Geometry.- Molecular Weight of the Chlorophyll Macromolecule.- 6 Environment.- Light ? Darkness.- Light and the Effectiveness of the Spectrum for Pigment Synthesis.- Physical Factors other than Light.- Chemical Environments.- Drug and Radiation Effects.- Environmental Effects on Chemical Analysis.- 7 Photosynthesis, Respiration, and Photochemical Activity.- Comparative Photosynthesis.- CO2 Fixation.- Respiration and Photosynthesis.- Cytochromes.- 8 Experimental Model Systems.- Chloroplastin.- Photochemical Activity.- Structure and Energy Transfer.- Other Model Structures.- 9 Motor Activity.- Photomotion.- Flagellar Motion.- 10 Summary.- Appendix: Media for Growth of Euglena.- References.- Author Index.