Metastasis Research Protocols

ISBN-13: 9781461482437 / Angielski / Twarda / 2013 / 250 str.

Miriam Dwek; Udo Schumacher; Susan A. Brooks

Opis

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Spis treści

1. In Vitro Invasion Assay Using Matrigel^TM - a  Reconstituted Basement Membrane Preparation

Debbie M S Hall and Susan A Brooks

 

2. Single Cell and Spheroid Collagen Type I Invasion Assay

Olivier De Wever , An Hendrix, Astrid De Boeck, Frank Eertmans, Wendy Westbroek, Geert Braems, Marc Bracke

 3. Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells

Deepashree Bapu, Munira Khadim and Susan A. Brooks

 

4. Small Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin under Physiological Flow Conditions

Ulrich Richter and Udo Schumacher

 

5. Adhesion of Tumour Cells to Matrices and Endothelium

Clara M Yates, Helen M McGettrick, Gerard B Nash, and G. Ed Rainger

 

6. Cell Aggregation Assays

Delphine Debruyne, Tom Boterberg, and Marc E. Bracke

 

7. Chick Heart Invasion Assay

Marc E. Bracke, Virinder S. Parmar, Anthony L. DePass, Christian V. Stevens, Barbara W. Vanhoecke, and Marc M. Mareel

 8. Computer Simulation of the Metastatic Progression

Gero Wedemann, Anja Bethge, Volker Haustein, and Udo Schumacher

 

9. Theoretical Considerations In Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice

Sue A. Watson and Rajendra Kumari

 10. Syngeneic Murine Metastasis Models: B16 Melanoma

Raffaella Giavazzi and Alessandra Decio

 

11. Imageable Clinically-relevant Mouse Models of Metastasis

Robert M. Hoffman

 

12. Imaging Metastatic Cell Trafficking at the Cellular Level in vivo with Fluorescent Proteins

Robert M. Hoffman

 

13. Ultrasound Techniques for the Detection of Tumours and Metastases in Small Animals

Michael Didié and Wolfram-Hubertus Zimmermann

 

14. The PFP/RAG2 Double Knock Out Mouse in Metastasis Research: Small Cell Lung Cancer and Prostate Cancer

Imke Müller and Sebastian Ullrich

 

15. Ultrasound Guided Intracardial Injection and In-vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Haematogenous Metastases

Johannes Salamon and Kersten Peldschus

 

16. Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer

Kersten Peldschus and Harald Ittrich

 

17. Spontaneous and Experimental Metastasis Models: Nude Mice

Janet E Price

 

18. Identifying the Origin and Phenotype of Cells in Tumour Xenografts

Rosemary Jeffery, Pooja Seedhar, and Richard Poulsom

 

Blurb

The second edition of Metastasis Research Protocols brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of  the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Metastasis Research Protocols, Second Edition: Volume 2 seeks to aid scientists in the further study of new methods in the area of metastasis research.