Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols

ISBN-13: 9780896038516 / Angielski / Twarda / 2002 / 356 str.

Donald Armstrong

Opis

In our first protocols book, Free Radical and Antioxidant Protocols (1), r- erence to in vivo, ex vivo, or in situ techniques were few compared to classical biochemical assays and only 6 of the 40 chapters were concerned with these applications. In our second book, Oxidative Stress Biomarkers and Antioxidant Protocols (2), which is being published concurrently with this third volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, the number of such chapters has increased. The literature dealing with histoche- cal/cytochemical and immunohistochemical techniques and staining to identify cellular/subcellular sites of oxidative stress has expanded rapidly, as has the molecular biology methodology used to analyze free radical and antioxidant (AOX) reactions, as well as the monitoring of living tissue. A two-way search was performed for each technique listed in Table 1, coupled with "oxidative stress" using the PUBMED search engine from the National Library of Medicine at NIH. Most of the techniques involved in m- suring oxidative stress employ molecular biology or ultrastructural approaches. Of these techniques, histology, polymerase chain reaction, and Western blotting are the most widely used. Several forms of therapy are now available for patients with increased oxidative stress. In addition to standard antioxidant therapy supplementation in vivo and in vitro, photodynamic therapy (PDT) employs excitation of a photon-emitting compound delivered systemically for free radical-mediated necrosis of affected tissues, and stem cells are also being used to induce signaling events or replace antioxidant enzymes.

Recenzje

"...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement." - E-STREAMS

Spis treści

Part I. Techniques for Histological Identification Cytochemical Localization of H2O2 in Biological Tissues E. Ann Ellis and Maria B. Grant Localization of Intracellular Lipid Peroxides Using Tetramethylbenzidine Reaction for Transmission Electron Microscopy E. Ann Ellis, Shigehiro Iwabuchi, Don Samuelson, and Donald Armstrong The Immunohistochemical Localization of Glutathione Peroxidase Kiyoshi Akeo, Tadahisa Hiramitsu, and Keiichi Watanabe 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody Hideyuki J. Majima, Takako Nakanishi-Ueda, and Toshihiko Ozawa Immunohistochemical Detection of Protein Oxidation Jürgen Frank, Alfonso Pompella, and Hans K. Biesalski Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells Alfonso Pompella, Silvia Dominici, Jürgen Frank, and Hans K. Biesalski Part II. Techniques for Subcellular Localization Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts (AGE Receptors) Paul J. Thornalley Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranes Kiyomi Kikugawa X-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative Stress R. Preston Mason and Robert F. Jacob Detection of Estrogen Receptor by In Situ Hybridization Kaori Kobayashi and Hiroshi Kobayashi Tumor Necrosis Factor-a Quantification and Expression by In Situ Hybridization Tracey A. Ignatowski and Robert N. Spengler Part III. Techniques for Molecular Biology Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells Ahmad Aljada, Husam Ghanim, and Paresh Dandona Activation of Nuclear Factor-kB (NF-kB) in Mononuclear Cells (MNC) Ahmad Aljada, Husam Ghanim, and PareshDandona Assay of Intracellular Hydrogen Peroxide Generation in Activated Individual Neutrophils by Flow Cytometry Yoshikazu Ito and David A. Lipschitz DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs) Gieri Camenisch, Roland H. Wenger, and Max Gassmann Analysis of Heat-Shock Transcription Factor and Element-Binding Activity Yong J. Lee Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation Enbo Ma and Gabriel G. Haddad An Expression System for a Transporter of Iron and Other Metals Michael D. Garrick and Kevin G. Dolan Analysis of Gene Expression Following Oxidative Stress Dana R. Crawford, Toshihide Suzuki, Jan Sesay, and Kelvin J. A. Davies Measurement of Immunoglobulin G Oxidation by Western-Blot Analysis Andrew Chow, Shahid Ahmed, Larrisa Chaplia, and Joseph Mattana Thioredoxin and Redox Regulation of the Nuclear Receptor Yuichi Makino, Kensaku Okamoto, and Hirotoshi Tanaka Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA Xin Gen Lei and Wen-Hsing Cheng Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase Kunio Yagi, Sadaaki Komura, and Nobuko Ohishi Part IV. Techniques for In Vivo/Ex Vivo/In Vitro Applications Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method Takashi Sakurai and Susumu Terakawa In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy David M. Hall and Garry R. Buettner Spectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Biopsies at 9.5 GHz Jürgen Fuchs, Norbert Groth, and Thomas Herrling In Vivo/In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy Pei Tsai, Supatra Porasuphatana, Howard J. Halpern,

Blurb

It is now feasible to identify subcellular sites where oxidative stress reactions occur and to determine gene expression relevant to those complex pathways. In Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, a panel of world authorities present cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, X-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47phox translocation, and microsphere delivery systems. The collection emphasizes the visual observation of free radicals or products altered by oxidative stress, and includes numerous in vivo, ex vivo, and in vitro applications. Two companion volumes, Oxidative Stress Biomarkers and Antioxidant Protocols (2002), and Free Radical and Antioxidant Protocols (1998), focus on the related biochemical and cellular biological methods.
Authoritative and highly practical, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols provides a comprehensive collection of powerful ultrastructure and molecular tools for the evaluation of metabolic disorders linked to oxidative stress and the aging process.