Neural Transplantation Methods

ISBN-13: 9780896037939 / Angielski / Twarda / 1999 / 556 str.

Stephen B. Dunnett; Glen B. Baker; Alan A. Boulton

Opis

we might seek alternative sources of donor tissues. Genetic en- neering, expansion of precursor cells, generation of immortalized cell lines, and transplantation between species are all under active investigation. Although significant difficulties remain for each of these alternatives, the problems appear soluble and relevant knowledge is expanding rapidly. As we enter the twenty-first century, the place of neural tra- plantation in experimental neuroscience is continuing to evolve. Rather than being a topic in its own right, neural transplantation increasingly serves as just another technique in the researcher's armory--alongside lesions, pharmaceuticals, gene transfer, and a variety of other techniques--for the experimental manipulation of brain structure and function. This is particularly true for studies of degeneration, plasticity, regeneration, and recovery of function in the nervous system, topics of increasing importance as experimental neurobiology is required to serve the higher needs of neurological and mental health in aging societies. Within this evolving context, Neural Transplantation Methods seeks to serve a particular need: to provide experimental neu- scientists with a source book of information to enable them to select and adapt transplantation techniques to their own expe- mental programs. All authors have been asked to address prac- cal issues, to enable the reader to assess what is available, what are the alternatives, what are the practical issues to be resolved in applying a particular protocol and getting it to work reliably in their unique experimental context.

Recenzje

"...a book with so much information is very valuable for neuroscientists."- Clinical Neurology and Neurosurgery

"Neural Transplantation Methods seeks to serve a particular need: to provide experimental neuroscientists with a source book of information to enable them to select and adapt transplantation techniques to their own experimental programs....Neural Transplantation Methods is a very interesting, up-to-date, and complete book on the technical and methodological issues in relation with experimental neural transplantation. It is a perfect source book of information for those who want to include the transplantation strategy in their experimental program." - Acta Neurologica Belgica

Spis treści

Sources of Cells for Transplantation. Dissecting Embryonic Neural Tissues for Transplantation, Stephen B. Dunnett and Anders Björklund. Pregraft Tissue Storage Methods for Intracerebral Transplantation, Hansjörg Sauer. Neural Stem Cells for Transplantation, Siddharthan Chandran and Clive N. Svendsen. Immortalized Cells, Vincent M. Mann and John D. Sinden. Cells from Testis for Transplantation in the CNS, Alison E. Willing and Paul R. Sanberg. Engineered Cells and Ex Vivo Gene Transfer, Cecilia Lundberg. Intracerebral Gene Transfer Using Viral Vectors, Ronald J. Mandel, Steve Hardy, Richard O. Snyder, Luigi Naldini, and Stuart E. Leff. Methods of Implantation. Basic Transplantation Methods in Rodent Brain, Stephen B. Dunnett and Anders Björklund. Intraocular Grafting, Ingrid Strömberg. Neural Transplantation in the Developing CNS, Anthony J. Castro and Jens Zimmer. Preparation of Cell Suspensions for Transplantation, Roger A. Barker, Anne E. Rosser, and Stephen B. Dunnett. Microtransplantation of Nigral Dopamine Neurons: A Step-By-Step Recipe, Guido Nikkhah, Christian Winkler, Alexandra Rödter, and Madjid Samii. Application of Polymer-Encapsulated Cell Therapy for CNS Diseases, Dwaine F. Emerich and Shelley R. Winn. Neural Transplantation in Parkinsonian Primates, Lucy E. Annett. Transplanting Myelinogenic Cells into the CNS, Robin J. M. Franklin and William F. Blakemore. Labeling and Identifying Grafted Cells, Alan R. Harvey. Factors in Graft Survival and Function. Neovascularization and the Blood-Brain Barrier in Neural Transplantation, Jeffrey M. Rosenstein. Trophic Influences on Neural Tissue Transplants: Delivery Methods and Co-graft Interaction, Ann-Charlotte Granholm. Neuroprotective Strategies in Neural Grafting, Patrik Brundin and Gabriele S. Kaminski Schierle. Death and Survival in CNS Grafting, James W. Fawcett. Immunology: Mechanisms of Rejection, Yoshinori Ohmoto and Kathryn Wood. Immunoprotection of Cell and Tissue Implants in the CNS, Colin Wattsand Stephen B. Dunnett. Immunobiology of Neural Xenotransplantation, Walter C. Low, Wei-Ming Duan, C. Dirk Keene, Hsiao-Tzu Ni, and Marcus A. Westerman. Index.

Blurb

In Neural Transplantation Methods, leading experimental neurobiologists and neurosurgeons provide all the technical and practical information required to perform neural transplantation in experimental and neurological research, including a detailed consideration of the issues involved in designing and customizing such methods for specific research purposes. The authors outline the different sources of donor tissues and other materials for implantation, and discuss the practicalities of embryonic staging and dissection, of the storage of cells by hibernation or cryopreservation, of the expansion of precursor cells, and of the genetic manipulation of cells for in vivo or ex vivo gene transfer. Tissue implantation techniques used for experimental animals of different species include: in oculo and demyelination models, microtransplantation of cell suspensions, encapsulation of transplanted cells, implantation into immature animals-especially primates-and labeling transplanted cells for subsequent detection. Special attention is given to the factors determining transplant survival and integration, detailing the role of the blood-brain barrier, the importance of vascularization for proper graft nutrition, the role of trophic factors, and strategies for immune suppression.
Neural Transplantation Methods constitutes the single most comprehensive sourcebook of practical information on the effective use of transplantation techniques in experimental neurobiology. Its powerful and flexible techniques will significantly aid all those now working to develop today's powerful new treatments of brain and spinal cord diseases and injuries.