Epitope Mapping Protocols

ISBN-13: 9780896033757 / Angielski / Miękka / 1996 / 416 str.

Glenn E. Morris

Opis

Interest in epitope mapping, or finding out where antibodies bind to their antigens, is by no means restricted to immunologists, but is shared by biolo gists from a wide range of disciplines in which antibodies are used as molecu lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune diseases, or in defining protein topology in intact cells or organelles, to mention only a few of the possibilities. The aim of Epitope Mapping Protocols is to provide both a useful range of alternative practical methods for the experienced mapper and a fairly com prehensive introduction for someone embarking on antibody production and mapping for the first time. Contributors were encouraged to illustrate their protocols with results from their own research and most of them elected to do so. After an introductory chapter, the protocols are arranged in three groups: The first group of twelve methods uses (or can use) whole, native antigens and may therefore be suitable for conformational epitopes; the second group of five uses peptides or peptide libraries; and the third group of eleven requires antigen expressed from recombinant DNA. Within this last group, methods more likely applicable to conformational epitopes are placed first."

Spis treści

Overview: Choosing a Method for Epitope Mapping Glenn E. Morris Crystallographic Studies of Antigen-Antibody Interactions Frederick A. Saul and Pedro M. Alzari Epitope Mapping Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy Irina Kustanovich and Anat Zvi Mapping Epitopes on Antigens by Immunodiffusion in Gel Giuseppe A. Molinaro and William C. Eby A Simple Solid-Phase Competition Assay with Labeled Antigen Masahide Kuroki Epitope Mapping by Antibody Competition: Methodology and Evaluation of the Validity of the Technique Socrates J. Tzartos Epitope Mapping by Surface Plasmon Resonance in the BIAcore Berit Johne Identifying Residues in Antigenic Determinants by Chemical Modification N. Martin Young and Raymond P. Oomen Epitope Mapping by Differential Chemical Modification of Antigens Hans Rudolf Bosshard Epitope Mapping by Proteolysis of Antigen-Antibody Complexes: Protein Footprinting Ronald Jemmerson Proteolytic Fragmentation for Epitope Mapping Maria R. Mazzoni, Nickolai O. Artemyev, and Heidi E. Hamm Epitope Mapping by Chemical Fragmentation Glenn E. Morris Probing Antibody-Antigen Interactions by Mass Spectrometry Yingming Zhao and Brian T. Chait Epitope Mapping Using Multipin Peptide Synthesis Stuart J. Rodda, N. Joe Maeji, and Gordon Tribbick SPOT Synthesis: Epitope Analysis with Arrays of Synthetic Peptides Prepared on Cellulose Membranes Ronald Frank and Heike Overwin Tea Bag Synthesis of Positional Scanning Synthetic Combinatorial Libraries and Their Use for Mapping Antigenic Determinants Clemencia Pinilla, Jon R. Appel, and Richard A. Houghten Epitope Mapping Using Phage-Displayed Peptide Libraries Diane Dottavio Reiterative Screening of Phage-Display Peptide Libraries, with Antibodies Alexander Pereboev and Glenn E. Morris Homolog Scanning Lin-Fa Wang Epitope Mapping Using an Oligonucleotide Replacement Method Hannah Alexander Epitope Mapping by Region-Specified PCR Mutagenesis Takehiko Shibata and Masayuki Ikeda Random Fragment Libraries Using Yeast Expression Plasmid Serge Bénichou and Geneviève Inchauspé Epitope Mapping Using Random Fragment Expression Libraries in l Phages Hartmut Porzig and Kenneth D. Philipson Random Fragment Libraries Displayed on Filamentous Phage Lin-Fa Wang and Meng Yu Epitope Mapping by Expression of Restriction Enzyme or PCR Fragments in Bacterial Plasmids Johannes A. Lenstra and Arnoud H. M. Van Vliet Epitope Mapping of Protein Antigens by Expression-PCR (E-PCR) David E. Lanar, Kevin C. Kain, and Henry B. Burch Epitope Mapping on Extracellular Domains of Cell-Surface Proteins Using Exonuclease III Thomas Brümmendorf, Antonius Plagge, and Ullrich Treubert An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis Steven G. Sedgwick, Brian A. Morgan, Nguyen thi Man, and Glenn E. Morris Incomplete Polypeptides of In Vitro Translation for Epitope Localization Lisa Djavadi-Ohaniance and Bertrand Friguet T-Cell Epitope Mapping with Synthetic Peptides and Peripheral Blood Mononuclear Cells Stuart J. Rodda Use of Natural or Selected Mutants and Variants for Epitope Mapping Glenn E. Morris Production of Panels of Monoclonal Antibodies by the Hybridoma Method Nguyen thi Man and Glenn E. Morris Production of Phage-Display Antibodies for Epitope Mapping Jenny Walker and George Banting Index