Patrick Thayer, Hector Martinez, and Erik Gatenholm
2. Cell Processing for 3D Bioprinting: Quality Requirements for Quality Assurance in Fundamental Research and Translation
Jeremy M. Crook
3. Computer-Aided Design and Manufacturing (CAD/CAM) for Bioprinting
Cormac D. Fay
4. Ethics and Policy for Bioprinting
Eliza Goddard and Susan Dodds
5. Extrusion Based Bioprinting: Current Standards and Relevancy for Human-Sized Tissue Fabrication
Kelsey Willson, Dongxu Ke, Carlos Kengla, Anthony Atala, and Sean V. Murphy
6. Stereolithography 3D Bioprinting
Hitendra Kumar and Keekyoung Kim
Part II Protocols for 3D Bioprinting
7. Characterizing Bioinks for Extrusion Bioprinting: Printability and Rheology
Cathal D. O’Connell1, Junxiang Ren, Leon Pope, Yifan Li, Anushree Mohandas, Romane Blanchard, Serena Duchi, and Carmine Onofrillo
8. Laser-Assisted Bioprinting for Bone Repair
Davit Hakobyan, Olivia Kerouredan, Murielle Remy, Nathalie Dusserre, Chantal Medina, Raphael Devillard, Jean-Christophe Fricain, and Hugo Oliveira
9. Bioprinting Stem Cells in Hydrogel for In Situ Surgical Application: A Case for Articular Cartilage
Serena Duchi, Carmine Onofrillo, Cathal O’Connell, Gordon G. Wallace, Peter Choong, and Claudia Di Bella.
10. 3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues
Eva Tomaskovic-Crook and Jeremy M. Crook
11. 3D Coaxial Bioprinting of Vasculature
Yang Wu, Yahui Zhang, Yin Yu, and Ibrahim T. Ozbolat
12. Principles of Spheroid Preparation for Creation of 3D Cardiac Tissue Using Biomaterial-Free Bioprinting
Chin Siang Ong, Isaree Pitaktong, and Narutoshi Hibino
13. Bioprinting for Human Respiratory and Gastrointestinal In Vitro Models
Manuela Estermann, Christoph Bisig, Dedy Septiadi, Alke Petri-Fink, and Barbara Rothen-Rutishauser
14. Bioprinting for Skin
Cristina Quílez, Gonzalo de Aranda Izuzquiza, Marta García, Verónica López, Andrés Montero, Leticia Valencia, and Diego Velasco
15. 3D Bioprinting and Differentiation of Primary Skeletal Muscle Progenitor Cells
Catherine Ngan, Anita Quigley, Cathal O’Connell, Magdalena Kita, Justin Bourke, Gordon Wallace, Peter Choong, and Robert M.I. Kapsa
16. Bioprinting Strategies for Secretory Epithelial Organoids
Ganokon Urkasemsin, Sasitorn Rungarunlert, and João N. Ferreira
17. Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling
Jeremy M. Crook and Eva Tomaskovic-Crook
This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.