3D Sponge-Matrix Histoculture: Methods and Protocols

ISBN-13: 9781493992720 / Angielski / Miękka / 2019 / 232 str.

Robert M. Hoffman

Opis

This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam (R) histoculture; development of the histoculture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam (R) histoculture of skin as well as isolated hair follicles in Gelfoam (R) histoculture; the use of Gelfoam (R) histoculture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam (R) histoculture. The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture.

Spis treści

Dedication…

Preface…

Table of Contents…

Epilogue…

Afterword…

1. In Memorium: Joseph Leighton, 1921-1999 – Father of 3-Dimensional Tissue Culture

Robert M. Hoffman

2. 3D Sponge-Matrix Histoculture: An Overview

Robert M. Hoffman

3. In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture

Robert M. Hoffman and Aaron E. Freemnan

4. Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology

Robert M. Hoffman and Fiorella Guadagni

5. Development of the Histoculture Drug Response Assay (HDRA)

Robert M. Hoffman and Robert A. Vescio

6. Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture

Robert M. Hoffman

7. Clinical Correlation of the Histoculture Drug Response Assay in Gastrointestinal Cancer

Robert M. Hoffman

8. Prospective Clinical Correlation of the Histoculture Drug Response Assay for Ovarian Cancer

Robert M. Hoffman, Phill-Seung Jung, Moon-Bo Kim, and Joo-Hyun Nam

9. Clinical Correlation of the Histoculture Drug Response Assay for Head and Neck Cancer

Robert M. Hoffman

10. Clinical Usefuleness of the Histoculture Drug Response Assay for Breast Cancer

Robert M. Hoffman and Hirokazu Tanino

11. Clinical Usefuleness of the Histoculture Drug Response Assay for Prostate Cancer and Benign Prostate Hypertrophy (BPH)

Robert M. Hoffman

12. In Vivo-Like Cell Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real-Time by FUCCI Imaging

Robert M. Hoffman and Shuya Yano

13. Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture

Robert M. Hoffman

14. Hair-Shaft Growth in Gelfoam® Histoculture on Skin and Isolated Hair Follicles

Robert M. Hoffman and Lingna Li

15. Histoculture of Hair-Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair

Fang Liu and Robert M. Hoffman

16. Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid

Robert M. Hoffman, Sumiyuki Mii, Jennifer Duong, and Yasuyuki Amoh

17. Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam®

Andrea Introini, Wendy Fitzgerald, Christophe Vanpouille, and Leonid Margolis

18. Imaging DNA Repair after UV Irradiation Damage of Cancer Cells in Gelfoam® Histoculture

Shinji Miwa and Robert M. Hoffman

19. Comparison of “Dimensionality” of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel

Yaunori Tome, Fuminari Uehara, Fuminori Kanaya, and Robert M. Hoffman

20. Imaging the Governing Step of Metastasis in Gelfoam® Histoculture

Robert M. Hoffman and Takashi Chishima

O autorze

Robert M. Hoffman, Ph.D. Professor of Surgery, University of California, San Diego President, AntiCancer, Inc. 7917 Ostrow Street San Diego, CA 92111, USA

Blurb

This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam® histoculture; development of the histoculture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam® histoculture of skin as well as isolated hair follicles in Gelfoam® histoculture; the use of Gelfoam® histoculture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam® histoculture.  The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting–edge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture.