Part I: Standard 2D culture on plastic 1. 2D culture on plastic, past, present, important findings and breakthroughs 2. The importance of microenvironment (ECM, GAG)
Part II: 3D lung models 3. ALI model 4. Biofilms 5. Lung organoid models 6. 3D models Artificial material 7. 3D extracellular matrix models 8. Lung-on-a-chip 9. Flow, Mechanical properties, and bioreactors 10. Challenges with 3D models and Future directions
Part III: New directions 11. 3D printing and bioprinting 12. Drug-screening and high throughput in 3D lung models 13. Model validation 14. AI and computational modelling 15. Conclusions