Virus-Host Interactions: Methods and Protocols

ISBN-13: 9781493963089 / Angielski / Miękka / 2016 / 372 str.

Susanne M. Bailer; Diana Lieber

Opis

This volume covers various aspects of virological research, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level. It features step-by-step, readily reproducible protocols and key implementation advice.

Spis treści

1. A High-Throughput Yeast Two Hybrid Protocol to Determine Virus-Host Protein Interactions

            Hannah Striebinger, Manfred Kögl, and Susanne M. Bailer

 

2. Analysis of Protein-Protein Interactions Using LUMIER Assay

Sonja Blasche and Manfred Koegl

 

3. Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation

            Stefan Becker and Jens von Einem

 

4. Discovery of Host-Viral Protein Complexes During Infection

Daniell L. Rowles, Scott S. Terhune, and Ileana M. Cristea

 

5. Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi

            Samantha J. Griffiths

 

6. High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay by Metabolic Labelling of Newly Transcribed RNA using 4-thiouridine

            Lars Dölken

 

7. Isolation and Characterization of Pathogen-Bearing Endosomes Enables Identification of New Cellular Cofactors and Analysis of Endosomal Escape

            Konstanze D. Scheffer, Ruth Popa-Wagner and Luise Florin

 

8. Computational Analysis of Virus-Host Interactomes

            Caroline C. Friedel

 

9. Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins

            Shuai Wang, Kezheng Wang, and Chunfu Zheng

 

10. Detection of Integrated Herpesvirus Genomes by Fluorescent in situ Hybridization (FISH)

            Benedikt Kaufer

 

11. Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes

            Jens Bernhard Bosse, Jessica Ragues, and Harald Wodrich

 

12. Determination of HSV-1 Infectivity Using a Luciferase Reporter Cell Line

            Diana Lieber and Susanne M. Bailer

 

13. Generation of a Cell Line for Constitutive Expression of Host or Viral miRNAs

            Diana Lieber

 

14. Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry   

            Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger

 

15. High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real-Time

            Ruben Martinez, Andrew M. Burrage, Christopher M. Wiethoff, and Harald Wodrich

 

16. Three-Dimensional Visualization of Virus Infected Cells by Serial Sectioning - An Electron Microscopic Study Using Resin Embedded Cells

            Martin Schauflinger, Clarissa Villinger, and Paul Walther

 

17. 3D Tissue Model for Herpes Simplex Virus-1 Infections

            Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer

 

18. In vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI)

            Wali Hafezi and Verena Hoerr

 

19. Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry

            Tina Schmidt and Martina Sester

 

20. Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells

            Rebecca J. Aicheler and Richard J. Stanton

 

21. Assessment of Natural Killer Cell Responses to Human Cytomegalovirus Infected Macrophages

            Zeguang Wu, Giada Frascaroli, and Thomas Mertens

 

22. Analysis of Cell Migration During HCMV Infection

            Stefania Varani and Giada Frascaroli

 

23. Gene Targeting in Mice: A Review

            Hicham Bouabe and Klaus Okkenhaug

 

24. A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant ES Cells

            Hicham Bouabe and Klaus Okkenhaug

 

25. Measurement of Mouse Cytomegalovirus Induced Interferon-β with Immortalized Luciferase Reporter Cells

            Evgenia Scheibe, Stefan Lienenklaus, Tobias May, Vladimir Goncalves Magalhaes, Siegfried Weiss, and Melanie M. Brinkmann

Blurb

Virus-Host Interactions: Methods and Protocols covers various aspects of virological research, such as biochemical approaches, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level with a strong focus on the manifold possibilities to study protein-protein interactions, as well as cell biological and immunological methodologies. Viruses represent a reduced form of life that depends on host cells for propagation. To this end, viruses approach and penetrate cells and usurp cellular machineries for their own benefit. Recent technological improvements have enabled the systematic analysis of the virus-host interplay be it on the genomic, the transcriptomic, or proteomic level. In parallel, bioinformatic tools have emerged in support of the large datasets generated by these high-throughput approaches. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Virus-Host Interactions: Methods and Protocols will prove invaluable to professionals and novices with its well-honed methodologies and protocols.