T-Cell Development: Methods and Protocols

ISBN-13: 9781071627396 / Angielski / Twarda / 2022 / 384 str.

Remy Bosselut; Melanie S. Vacchio

Opis

This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.

Spis treści

Part I: Background Information

1             A beginner’s guide to T cell development

Rémy Bosselut

 

2          Revelations in thymic epithelial cell biology and heterogeneity from single cell RNA sequencing and lineage tracing methodologies

Abigail Morales-Sanchez^, Susannah C Shissler, Jennifer E Cowan, and Avinash Bhandoola

 

3          Early development of innate lymphoid cells

Yi Ding, Christelle Harly, Arundhoti Das and Avinash Bhandoola

 

4          Development of gd T cells, Soldiers on the Front Lines of Immune Battles

Alejandra V. Contreras and David L. Wiest

 

5          Principles of Advanced Flow Cytometry: A Practical Guide

Shafiuddin Siddiqui and Ferenc Livák

 

 

Part II: Core Approaches and Strategies

 

6          Genetic strategies to study T cell development

Rémy Bosselut

 

7          A simplified approach to evaluating T cell development by flow cytometry

            Jan Y.M. Lee and Paul E. Love

 

8          Purification of thymocyte and T cell subsets

Andrea C. Carpenter, Laura B. Chopp, Jong Kyong Kim and Rémy Bosselut

 

9              Generation of bone marrow chimeras

Yayi Gao and Rémy Bosselut

 

10        Generation of murine T cell effector populations in vitro

Melanie S. Vacchio

 

Part III: Specific Techniques

 

11      Large-scale isolation of mouse thymic epithelial cells

Izumi Ohigashi, Mami Matsuda-Lennikov, and Yousuke Takahama

 

12        Generation of retrogenic mice to investigate T cell development

Yayi Gao and Rémy Bosselut

 

13        Purification of bone marrow precursors to T cells and ILCs.

Devin Kenney and Christelle Harly

 

14        Studying T cell Development in Neonatal and Adult Thymic Slices

Laura L. McIntyre, Lydia K. Lutes and Ellen A. Robey

 

15        Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines

Mahmood Mohtashami, Patrick Brauer, and Juan Carlos Zúñiga-Pflücker

 

16        Methods for Study of Mouse T Cell Receptor a and b Gene Rearrangements

Danielle J. Dauphars, Glendon Wu, Craig H. Bassing^,, and Michael S. Krangel

 

17        Ultrasound guided intra-thymic cell injection

Hristo Georgie, Laura B. Chopp and Kristin A. Hogquist

 

18        Fetal thymic organ culture and negative selection

Emma Teixeiro, Mark A. Daniels

 

19        In Vitro Analysis of Thymocyte Signaling

Emma Teixeiro, and Mark A. Daniels

 

20        Identification and purification of human T cell precursors

Inge Van de Walle, Nina Lambrechts, Anaïs Derveeuw, Marieke Lavaert, Juliette Roels and Tom Taghon

 

21        In vitro model systems to study human T cell development

Nina Lambrechts, Kai Ling Liang, Imke Velghe, Steven Strubbe, Anne-Catherine Dolens and Tom Taghon

 

22     Zebrafish: A Tractable Model for Analysis of T cell Development

         Robert Sertori, Yong Zhang and David L. Wiest