Dengue is a tropical, mosquito borne flavivirus infection and a leading public health problem in India. Four serotypes DEN1-4 cause high morbidity and mortality. Dengue is a spherical, lipid enveloped, positive stranded RNA virus having a 10200 Kb RNA genome coding for three structural (capsid C, premembrane PrM, and envelope E) and seven nonstructural proteins. Early, sensitive and specific diagnosis is paramount for patient management, prevention of complications, etiologic investigation and disease control. Early diagnosis is achieved by NS1 antigen detection, nucleic acid amplification...