Biofilm is an association of microorganisms in which cells stick to each other on a surface encased within matrix of extracellular polymeric substance produced by bacteria themselves. Periodontal disease being biofilm-mediated disease, it is widely accepted that the conversion from periodontal health to disease accompanies a shift in the indigenous ora of the plaque biofilm from gram-positive facultative to gram-negative anaerobic motile microorganisms.Its clinical importance and accessibility for in vivo research make dental plaque one of the most studied and best-understood biofilm...