The use of artificial reproduction techniques and cryopreservation biotechnology can support species conservation. Gametes and embryos cryopreservation and banks of frozen germplasm can be used in species conservation programs and genetic resource for "in situ" and "ex situ" populations. However, environmental factors, such as type of enclosure, number of animals per enclosure and animal-keeper interactions, therefore stress, diseases, nutritional deficiencies, genetics and behavioral alterations may affect semen and oocytes quality in order to be important for successful feline ...