This title constitutes the proceedings of the 5th International Conference on Typed Lambda Calculi and Applications, TLCA 2001, held in Krakow, Poland in May 2001. The 28 revised full papers presented were carefully reviewed and selected from 55 submissions. The volume reports research results on all current aspects of typed lambda calculi. Among the topics addressed are type systems, subtypes, coalgebraic methods, pi-calculus, recursive games, various types of lambda calculi, reductions, substitutions, normalization, linear logic, cut-elimination, prelogical relations, and mu calculus.