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As a part of the systematic and proteomic approach based characterization of plant material with the advantage of producing valuable informations of functional genomics for a general crop improvement programme, leaves of holy medicinal-aromatic plant Tulsi (Ocimum basilicum L.) were used as a source of proteins and a model eukaryotic system. In this study, we developed a reliable protocol for PEG mediated enrichment of low abundant cellular proteins to increase their detection through 2 DE-SDS PAGE. I6% PEG was found to be optimum for the precipitation of Ocimum protein. Further, proteome...

The present study reveals a part of the systemic annotation which includes the use of a proteomic tool with PEG mediated fractionation of plant proteome proteins for characterizing the explant(s) in order to study functional genomic approaches in tissue culture based sugarcane improvement programme. Stem segments derived in vitro propagated sugarcane leaves were used, for selection of salt tolerant callus lines and also for proteomic analysis.