Toxicogenomics: Principles and Applications

ISBN-13: 9780471434177 / Angielski / Twarda / 2004 / 362 str.

Cynthia A. Afshari; Hisham K. Hamadeh

Opis

Toxicogenomics: Principles and Applications fills the need for a single, thorough text on the key breakthrough technologies in genomics, proteomics, metabolomics, and bioinformatics, and their applications to toxicology research. The first section following a general introduction is on genomics and toxicogenomics, and qPCR. The next sections are toxicoproteomics and metabolomics. The final section covers bioinformatics aspects, from databases to data integration strategies.
A practical resource for specialists and non-specialists alike, this book includes numerous illustrations that support the textual explanations. It offers practical guidance to investigators wishing to pursue this line of research, and lists key relevant software and Internet resources.

Recenzje

"The editors have assembled a useful guide for new and seasoned scientists exploring the relationships between genetic variation and toxic response." ( Journal of Natural Products, January 2006)

" a comprehensive, concise, and practical introduction to this new and exciting area of toxicogenomics." (ASM News, December 2005)

" both skilled practitioners and newcomers to this subject will find something worthwhile and interesting within the covers of this volume." (The Quarterly Review of Biology, September 2005)

"...a timely and thorough compendium covering various subjects in toxicology, genetics, expression profiling, and bioinformatics...a valuable collection on new approaches " (Clinical Chemistry, August 2005)

The editors of this book are to be congratulated for their valuable arrangement of the expanding field of toxicogenomics, through its principles and application.   (Journal of Chemical Technology & Biotechnology, June 2005)

"Most suitable for genomic scientists learning about this new inter–disciplinary field of toxicogenomics." (E–STREAMS, March 2005)

"...an up–to–date guide into what is happening in this dynamic field...a useful roadmap to conduct new studies and to evaluate the increasing number of research papers." (Veterinary & Human Toxicology, December 2004)

"This timely book is well referenced, with particular attention to web–based resources. The emphasis on methods rather than signatures reveals the still–early nature of this promising field." (Environmental Health Perspectives, November 2004)

Spis treści

Contributors.

Foreword.

Preface.

1. General Toxicology (Ronald C Shank).

2. A Short Introduction to the Expression Profile Toolbox, (Jeffrey F. Waring, et al.).

3. Microarray Manufacture (Emile F Nuwaysir).

4. Scanners (Charles J Tucker).

5. Databases for Toxicogenomics (Pierre R Bushel).

6. Statistics for Toxicogenomics (Thomas J Downey Jr).

7. Real–time and Quantitative PCR (Nigel J Walker and Jeanelle Martinez).

8. Toxicogenomics: Confounding Variables and Data Interpretation (Kevin Morgan, et al.).

9. Introduction ito relationships between toxicology and gene expression (Hisham Hamadeh and Robert Dunn).

10. The Use of a Compendium of Expression Profiles for Mechanism of Toxicity Prediction (Jeffrey F Waring, et al.).

11. Using Genetically–Altered Mice in Toxicogenomics Analysis of Chemical Exposure (J Christopher Corton, et al.).

12. Introduction to High–Throughput Prot ein Expression (Alex Merrick).

13. Analytical Proteomics Approaches To Analysis Of Protein Modifications : Tools For Studying Proteome–Environment Interactions (Daniel Liebler).

14. Introduction to Metabolomics and Metabolic Profiling (Robert London and David Houck).

15. Toxicogenomics Resources (Hisham Hamadeh and Rupesh Amin).

Index.

O autorze

Dr. Hisham K. Hamadeh is a research scientist at Amgen. He earned his Ph.D. in toxicology from the University of California at Irvine, following up as a research fellow in the National Center for Toxicogenomics Microarray Center, NIEHS. He has received research excellence awards from the Society of Toxicology and American Cancer Society.

Dr. Cynthia A. Afshari is Associate Director of Toxicology at Amgen. She has served as Director of Basic Research Applications at the Microarray Center and Head of the Gene Regulation Group, Laboratory of Molecular Carcinogenesis, NIEHS. She has also held a position as adjunct professor for the Curriculum in Toxicology at the University of North Carolina, Chapel Hill.

Blurb

With the sequencing of human and other genomes completed, the new field of toxicogenomics is now emerging into the realm of practice. This field brings together many different disciplines to incorporate current genomics knowledge into traditional toxicology. By finding more precise ways to measure the response of living systems and environments to chemical exposures, toxicogenomics promises to greatly alter and improve the drug development process, to name just one area of application.

Toxicogenomics: Principles and Applications provides a unified, thorough text on key breakthrough technologies in genomics, proteomics, metabonomics, and bioinformatics, as well as their applications to toxicology research. Coverage includes:

• A general introduction to toxicology for nonspecialists
• Microarray/high–density gene expression profiling
• Quantitative RNA measurement
• Analysis of metabolites (metabonomics)
• Statistics and databases for toxicogenomics
• Functional genomics models
• Protein analysis and profiling

Throughout Toxicogenomics, the emphasis remains on information practitioners can put to use immediately. Numerous illustrations support the text, and a helpful summary lists key software and Internet tools, as well as additional resources. Written for specialists and nonspecialists alike, Toxicogenomics gives a wide range of researchers access to the unique combination of disciplines that characterize this exciting new field.