10. Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ
Shaheen Khan and Kelly A. Kaihara
11. High Throughput Single Cell Real-Time Quantitative PCR Analysis
Liora Haim-Vilmovsky
12. Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris™ System
Chad D. Sanada and Aik T. Ooi
13. Targeted TCR Amplification from Single-Cell cDNA Libraries
Shuqiang Li and Kenneth J. Livak
Part III Single Cell Genomic and Epigenomic Analysis
14. Sequencing the Genomes of Single Cells
Veronica Gonzalez-Pena and Charles Gawad
15. Studying DNA Methylation in Single Cell Format with scBS-seq
Natalia Kunowska
16. Single Cell 5fC Sequencing
Chenxu Zhu, Yun Gao, Jinying Peng, Fuchou Tang, and Chengqi Yi
17. ChIPmentation for Low-Input Profiling of In Vivo Protein-DNA Interactions
Natalia Kunowska and Xi Chen
Part IV Single Cell Proteomic Analysis
18. Immuno-Phenotyping of Human Peripheral Blood Mononuclear Cells by Mass Cytometry
Susanne Heck, Cynthia Jane Bishop, and Richard Jonathan Ellis
19. Classification of the Immune Composition in the Tumor Infiltrate
Davide Brusa and Jean-Luc Balligand
Part V Single Cell Multi Omic Analysis
20. Combined Genome and Transcriptome (G&T) Sequencing of Single Cells
Iraad F. Bronner and Stephan Lorenz
21. Simultaneous Pofiling of mRNA Transcriptome and DNA Methylome from a Single Cell
Youjin Hu, Qin An, Ying Guo, Jiawei Zhong, Shuxin Fan, Pinhong Rao, Xialin Liu, Yizhi Liu, and Guoping Fan
22. Simultaneous Targeted Detection of Proteins and RNAs in Single Cells
Aik T. Ooi and David W. Ruff
Part VI Single Cell Screening
23. CRISPR-Screening in Single Cells
Johan Henriksson
Part VII Single Cell Live Imaging
24. Single Cell Live Imaging
Toru Hiratsuka and Naoki Komatsu
Part VIII Single Cell Data Analysis
25. Differential Expression Analysis in Single Cell Transcriptomics
Luca Alessandri , Maddalena Arigoni, and Raffaele Calogero
26. A Bioinformatic Toolkit for Single-Cell mRNA Analysis
Kevin Baßler, Patrick Günther, Jonas Schulte-Schrepping, Matthias Becker, and Paweł Biernat
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This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.