Sertoli Cells: Methods and Protocols

ISBN-13: 9781493976973 / Angielski / Twarda / 2018 / 297 str.

Marco G. Alves; Pedro F. Oliveira

Opis

This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture.

Recenzje

"The pictures illustrating the book are all of high quality and the readers will be satisfied and certainly not disappointed while running through the chapters: exactly in the same manner you cannot stop of eating chapters or cherries, one is calling the other!" (CarloAlberto Redi, European Journal of Histochemistry, Vol. 63 (01), 2019)

Spis treści

1. Establishment of Primary Culture of Sertoli Cells

            Raquel L. Bernardino, Marco G. Alves, and Pedro F. Oliveira

 

2. Evaluation of the Purity of Sertoli Cell Primary Cultures

            Raquel L. Bernardino, Marco G. Alves, and Pedro F. Oliveira

 

3. Preparation of Testicular Samples for Histology and Immunohistochemistry

            Barbara Bilinska, Anna Hejmej, and Malgorzata Kotula-Balak

 

4. Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age

            Valeria Grieco and Barbara Banco

 

5. Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy

            Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Victor Quesada-Cubo, Concepción Ferrer, and Luis Miguel Pastor

 

6. Sertoli Cells Preparation for Co-Immunoprecipitation

            Maria João Freitas and Margarida Fardilha

 

7. Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation

            Maria João Freitas and Margarida Fardilha

 

8. Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy

            Marina G. Yefimova, Nadia Messaddeq, Annie-Claire Meunier, Anne Cantereau, Bernard Jegou, and Nicolas Bourmeyster

 

9. A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells

            Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka, and Yoichi Kondo

 

10. Assessing Autophagy in Sertoli Cells

            Chao Liu, Jehangir Khan, and Wei Li

 

11. Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells

            Luís Crisóstomo, Marco G. Alves, Agostina Gorga, Mário Sousa, María F. Riera, María N. Galardo, Silvina B. Meroni, and Pedro F. Oliveira

 

12. Assessing Sertoli Cells Metabolic Activity

            Ivana Jarak, Pedro F. Oliveira, Gustavo Rindone, Rui Carvalho, María Noel Galardo, María Fernanda Riera, Silvina Meroni, and Marco G. Alves

 

13. Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy

            Rita Ferreira, Fábio Trindade, and Rui Vitorino

 

14. Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs

            Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou, Weihui Zhang, and Zuping He

 

15. Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice

            Diane Rebourcet, Annalucia Darbey, Michael Curley, Peter O’Shaughnessy, and Lee B. Smith

 

16. Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons

            Qing Wen, Elizabeth I. Tang, Nan Li, Dolores D. Mruk, Will M. Lee, Bruno Silvestrini, and C. Yan Cheng

 

17. Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay

            Haiqi Chen, Wing-yee Lui, Dolores Mruk, Xiang Xiao, Renshan Ge, Qingquan Lian, Will M. Lee, Bruno Silvestrini, and C. Yan Cheng

 

18. Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell

            Pranitha Jenardhanan, Manivel Panneerselvam, and Premendu P. Mathur

 

19. A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells

            Anna Maggio, Raquel L. Bernardino, Patrizia Gena, Marco G. Alves, Pedro F. Oliveira, and Giuseppe Calamita

 

20. Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing

            Yoni Baert, Jaime Onofre, Dorien Van Saen, and Ellen Goossens

Blurb

This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.