Ribonucleases, Part A: Functional Roles and Mechanisms of Action: Volume 341

ISBN-13: 9780121822422 / Angielski / Twarda / 2001 / 734 str.

Allen W. Nicholson

Opis

This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and ribozymes.
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Spis treści

Section I: Ribonuclease Classification and Review

[1]: A Natural Classification of Ribonucleases

[2]: The Ribonuclease T1 Family

[3]: Ribonuclease T2

[4]: The Ribonuclease P Family

Section II: Ribunucleases Assays

[5]: Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity

[6]: Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method After Isoelectric Focusing

[7]: Gel Renaturation Assay for Ribonucleases

[8]: Analysis of Ribonucleases following Gel Electrophoresis

[9]: Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluorescence Correlation Spectroscopy

[10]: Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifically at a Chosen Site

[11]: Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNases

Section III: Secreted Ribonucleases

[12]: Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding Studies

[13]: Purification of Engineered Human Pancreatic Ribonuclease

[14]: Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases

[15]: Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms, Isoenzymes, Monomeric Forms; Assay for Selective Cytotoxicity of the Enzyme

[16]: Angiogenin

[17]: Eosinophil-Derived Neurotoxin

[18]: Eosinophil Cationic Protein

[19]: Deciphering the Mechanism of RNase T1

[20]: Mitogillin and Related Fungal Ribotoxins

[21]: RNase U2 and a-Sarcin: A Study of Relationships

[22]: Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill.

[23]: Leczyme

Section IV: Ribonucleases H

[24]: Prokaryotic Type 2 RNases H

[25]: RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature

[26]: Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae

[27]: Human RNases H

[28]: Assays for Retroviral RNase H

Section V: Synthetic Ribonucleases

[29]: Sequence-Selective Artificial Ribonucleases

[30]: RNA Cleavage by 1,4-Diazabicyclo[2.2.2]octane-Imidazole Conjugates

[31]: Preparation and Use of ZFY-6 Zinc Finger Ribonuclease

Section VI: Ribonucleolytic Nucleic Acids

[32]: RNA Cleavage by the 10-23 DNA Enzyme

[33]: Leadzyme

[34]: Hammerhead Ribozyme Structure and Function in Plant RNA Replication

[35]: Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme

[36]: Catalytic and Structural Assays for the Hairpin Ribozyme

[37]: Intracellular Applications of Ribozymes

Section VII: Ribonuclease Inhibitor

[38]: Barnase-Barstar Interaction

[39]: Cytoplasmic Ribonuclease Inhibitor

[40]: Small Molecule Inhibitors of RNase A and Related Enzymes

[41]: Ribonuclease-Resistant RNA Controls and Standards

Section VIII: Nonenzymatic Cleavage of RNA

[42]: Nonenzymatic Cleavage of Oligoribonucleotides

Author Index

Subject Index