Quantification of Tannins in Tree and Shrub Foliage: A Laboratory Manual

ISBN-13: 9789048164288 / Angielski / Miękka / 2010 / 102 str.

Harinder P. S. Makkar

Opis

Here is the most complete guide available for the analysis of tannins. A battery of tannin methodologies is presented in a simple, clear and easy-to-understand manner. This unique guide covers chemical, biological and radio isotopic tannin assays. Comprehensive step-by-step protocols are presented for each method. The protocols enable non-specialists and specialists alike to implement the methods easily in the laboratory. It is an ideal laboratory manual for research scientists, graduate students, and laboratory personnel working in the fields of animal nutrition, soil nutrient management, wild life-plant interactions, and plant breeding.

Spis treści

1. Chemical, Protein Precipitation and Bioassays for Tannins, Tannin Levels and Activity in Unconventional Feeds, and Efects and Fate of Tannins.- 2. Treatment of Plant Material, Extraction of Tannins, and an Overview of Tannin Assays Presented in the Manual.- 3. Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method.- 4. Determination of Condensed Tannins (Proanthocyanidins).- 5. Gallotannin Determination by Rhodanine Assay.- 6. Gallotannin Determination Using HPLC.- 7. Determination of Hydrolysable Tannins (Gallotannins and Ellagitannins) after Reaction with Potassium Iodate.- 8. Determination of Protein-Precipitable Phenolics.- 9. Protein-Binding Capacity by Filter Paper Assay.- 10. Radial Diffusion Assay.- 11. Tannin Bioassay.- 12. Radiolabelled BSA Precipitation Method.- 13. Simplified Radiolabelled BSA Precipitation Method.- 14. Characterization of Phenolic Compounds by Thin Layer Chromatography (TLC).- 15. References.- 16. A Summary of Results from the First Phase of the Coordinated Research Project.- 17. List of Participants in the Co-Ordinated Research Project.