1. Introduction to Protein Nanotechnology Laura J. Domigan and Juliet A. Gerrard
Part I Design and Production of Protein Building Blocks for Nanotechnology
2. Protein Expression in the Baculovirus-Insect Cell Expression System Adetayo A. Adeniyi and Linda Hwee-Lin Lua
3. Peroxiredoxin Proteins as Building Blocks for Nanotechnology Frankie Conroy and N. Amy Yewdall
4. Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins Sarah R. Ball, Chi L. L. Pham, Victor Lo, Ann H. Kwan, and Margaret Sunde
5. ATP Synthase: Expression, Purification, and Function Meghna Sobti, Robert Ishmukhametov, and Alastair G. Stewart
6. Molecular Superglues: Discovery and Engineering Orthogonalization Paul Young and Christopher Squires
7. Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes Samuel Lim, Douglas S. Clark, and Dominic J. Glover
Part II Towards Protein Nano-Devices
8. Adding Function to Protein Scaffolds Kyle Webster, Luigi Sasso, and Laura J. Domigan
9. Virus-Derived Nanoparticles Noor H. Dashti and Frank Sainsbury
10. Strategies for Increasing Protein Stability Peter G. Chandler, Sebastian S. Broendum, Blake T. Riley, Matthew A. Spence, Colin J. Jackson, Sheena McGowan, and Ashley M. Buckle
11. Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol Francesco Canfarotta, Sergey A. Piletsky, and Nicholas W. Turner
12. Nanotechnology with S-Layer Proteins Bernhard Schuster and Uwe B. Sleytr
Part III Meaningful Measurements of Protein Nanocomponents
13. Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy Lou Brillault and Michael J. Landsberg
14. Atomic Force Microscopy of Proteins Yiran An, Sesha Sarathchandra Manuguri, and Jenny Malmstrom
15. Native Protein Mass Spectrometry Timothy M. Allison and Mark T. Agasid
16. Nanoparticle Tracking Analysis of b-Casein Nanocarriers Hikmat Masri and Jared K. Raynes
17. Molecular Dynamics Simulation of Proteins Thomas A. Collier, Thomas J. Piggot, and Jane R. Allison
This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field.