Progranulin: Methods and Protocols

ISBN-13: 9781493993253 / Angielski / Miękka / 2019 / 363 str.

Andrew Bateman; Hugh P. J. Bennett; Siu Tim Cheung

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Introduction

1. A Brief Overview of Progranulin in Health and Disease

Andrew Bateman, Siu Tim Cheung, and Hugh P.J. Bennett

Part II Analytical and In Vitro Methods for the Study of Progranulin Biology

2. Chromatographic Methods for the Purification of Granulin Peptides

Andrew Bateman, Babykumari Chitramuthu, and Hugh P.J. Bennett

3. Methods for Expression and Purification of Biologically Active Recombinant Progranulin

Chun Yu Zhao and Andrew Bateman

4. Large-Scale Generation of Recombinant Granulin Peptides in E. coli

Dmitri Tolkachev

5. Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging

Dmitri Tolkatchev

6. Data Mining: Applying the AD&FTD Mutation Database to Progranulin

Marc Cruts and Christine Van Broeckhoven

Part III Methods to Investigate the Role of Prgranulin in Cancer

7. Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases

Ginette Serrero and David Hicks

8. Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker

Ginette Serrero and David Hicks

9. Analysis of Progranulin-Mediated Akt and MAPK Activation

Shi-Qiong Xu, Simone Buraschi, Ryuta Tanimoto, Manuela Stefanello, Antonio Belfiore, Renato V. Iozzo, and Andrea Morrione

10. Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models

Chi Wai Yip, Phyllis F.Y. Cheung, Nicholas C.L. Wong, Sze Wai Fung, and Siu Tim Cheung

11. Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features

Phyllis F.Y. Cheung and Siu Tim Cheung

12. Methods to Study the Role of Progranulin in the Tumor Microenvironment

Moshe Elkabets and Samuel Brook

Part IV Animal Models to Study the Role of Progragulin in Health and Disease

13. Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo using Caenorhabditis elegans

James Julian Doyle and J. Alex Parker

14. The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response

Tsung-Yuan Hsu, Victoria J. Butler, and Aimee W. Kao

15. Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function

Babykumari P. Chitramuthu and Hugh P.J. Bennett

16. Methods to Investigate the Molecular Basis of Progranulin Actions on Brain and Behavior In Vivo Using Knockout Mice

Jan Boddaert, Hans Wils, and Samir Kumar-Singh

17. Methods to Investigate the Protection Againts Neurodegenerative Disorders Provided by Progranulin Gene Transfer in the Brain

Jackalina M. Van Kampen

18. The Interaction between Progranulin with Sortilin and the Lysosome

Xiaolai Zhou, Peter M. Sullivan, Daniel H. Paushter, and Fenghua Hu

19. Methods to Study the Role of Progranulin in Preimplanatation Mouse Embryo Development

Laura Diaz-Cueto, Fabian Arechavaleta-Velasco, and George L. Gerton

20. Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-Inflammatory Activity of Progranulin in Inflammatory Arthritis

Jian-Iu Wei and Chuan-ju Liu

21. Methods for Studying the Function of Progranulin in Atherosclerosis using Both Knockout Mice Models and In Vitro Studies

Tohru Ohama, Ryota Kawase, Yasushi Sakata, and Shizuya Yamashita

22. Methods to Investigate the Roles of Prgranulin in Angiogenesis Using In Vitro Strategies and Transgenic Mouse Models

Huishi Toh, Eugene Daniels, and Andrew Bateman 

Blurb

This volume explores experimental strategies to study progranulin as a regulatory protein in biological and disease processes. The chapters in this book are divided into four parts: Part One is an introduction to the topic; Part Two looks at analytical and in vitro methods to study progranulin biology; Part Three discusses the role of progranulin in cancer; and Part Four describes the process of using animal models to study progranulin in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Progranulin: Methods and Protocols is a valuable resource for experienced researchers who want to expand their studies of this field, and newcomers who want to begin including progranulin into their work.”