12. Use of Fluorescent Reporters to Analyse Dynamic and Spatial Responses to Mechanical Wounding
Antoine Larrieu, Trang Hieu Nguyen, and Antony Champion
13. Quantitative Jasmonate Profiling using a High-Throughput UPLC-nanoESI-MS/MS Method
Cornelia Herrfurth and Ivo Feussner
14. Detection of Fungal Jasmonates by Liquid Chromatography Paired with Mass Spectrometry
Liu Yingyao, Rajesh N. Patkar, and Naweed I. Naqvi
15. CRISPR/Cas9-Mediated Gene Editing of the Jasmonate Biosynthesis OsAOC Gene in Rice
Hieu Trang Nguyen, Huong To Thi Mai, Daniel Moukouanga, Michel Lebrun, Stephane Bellafiore, and Antony Champion
16. Mass Spectrometric Approaches to Study the Metabolism of Jasmonates: Biotransformation of Exogenously-Supplemented Methyl Jasmonate by Cell Suspension Cultures of Moringa oleifera
Claude Y. Hamany Djande, Ntakadzeni E. Madala, and Ian A. Dubery
This second edition volume expands on the previous edition with a look at the latest techniques used to study plant hormone jasmonate (JA). The chapters in this book are organized into three parts: Parts One and Two discuss the role of JA in plant physiology and development, and in plant-biotic interactions. Part Three talks about methods used by researchers to study jasmonate metabolism and signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Jasmonate in Plant Biology: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field.