Immunofluorescence in Clinical Immunology: A Primer and Atlas

ISBN-13: 9783034895408 / Miękka / 2012 / 231 str.

Klein, R. A.

Opis

Immunofluorescence, a suitable laboratory method for the microscopic demonstration of antigens and antibodies in biological materials, useable, for example, to provide evidence for the pathogenesis of disease in histological or cytological preparations and for tumour cell differentiation. For this reason immunofluorescence has a decisive role as the method of choice for the diagnosis of auto-immune diseases. This primer on immunofluorescence techniques, which first appeared in 1979, is a richly illustrated handbook suitable for everyday practical work in the laboratory, useable as both an introduction to the subject as well as an atlas. In hardly any other area of medicine are there so many new findings to report. The second edition of this book is concerned not only with the detection methods which now form an essential and established part of diagnostic techniques, but also with the most recent research results such as the discovery of antibodies against Auerbach's plexus and against podocytes...

Spis treści

1 Introduction to the basic principles and techniques of immunofluor-escence.- 2 Production of antibodies and labelling with fluorochromes.- 3 Characterisation and quality control of antisera and conjugates.- 4 Actual optical immunofluorescence techniques.- 5 Instrumental technique and the documentation of findings.- 6 General advice.- 7 Applications of immunofluorescence.