Handbook of Vascular Biology Techniques

ISBN-13: 9789401797153 / Angielski / Twarda / 2015 / 477 str.

Mark Slevin; Garry McDowell

Opis

A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here.Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work.Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebrovascular, cancer and dementia.

Spis treści

Preface

Section 1 In vitro techniques

Chapter 1: In vitro angiogenesis assay - endothelial migration, proliferation, and tube formation

Chapter 2: Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis

Chapter 3: Induction of hypoxia in vascular endothelial cell culture

Chapter 4: Evaluating In Vitro Angiogenesis using Live Cell Imaging

Chapter 5: Isolation of Endothelial Progenitor Cells (EPCs)

Chapter 6: Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis

Chapter 7: Assessment of vascular function and contractility, ex vivo.

Chapter 8: Measurement of Aβ uptake by cerebrovascular smooth muscle cells

Chapter 9: Measurement of Intracellular Ca2+ in Human Endothelial cells

 

Section 2: In vivo and ex vivo manipulations

Chapter 10: Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion

Chapter 11: Sponge implant model of inflammatory angiogenesis

Chapter 12: The chick embryo chorioallantoic membrane assay

Chapter 13: Dorsal air sac assay

Chapter 14: Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals

Chapter 15: Hypoxia-induced retinal angiogenesis in adult zebrafish

Chapter 16: Angiogenesis in regenerating adult zebrafish tail fin

Chapter 17: Methods for studying developmental angiogenesis in zebrafish

Chapter 18: Isolation and Expansion of Brain Microvascular Endothelial Cells

 

Section 3: Imaging and histological analysis

Chapter 19: Adipose angiogenesis

Chapter 20: Assessing tumor angiogenesis in histological samples

Chapter 21: Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the  embryonic mouse skin and adult mouse cornea.

Chapter 22: Computed Tomography Angiography: Fundamental techniques and data interpretation

Chapter 23: Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation

Chapter 24: Single-Photon Emission Tomography of the brain in vascular pathology

 

Section 4: Miscellaneous novel techniques in vascular biology

Chapter 25: Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues

Chapter 26: Preparation of liposomes with dual fluorophores to follow real-time content release in vivo

Chapter 27: Vascular Flow Modelling using Computational Fluid Dynamics

Chapter 28: Reverse Transcription Real-Time PCR Protocol for gene expression analyses

Chapter 29: Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis

Chapter 30: Genomic microarray analysis

Chapter 31: Selection of appropriate housekeeping genes for quality control

Chapter 32: Endothelial transcriptomic analysis

Chapter 33: Protocol for Multiplex Amplicon Sequencing using Barcoded Primers

Chapter 34: Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells:  Identification and Quantification

Chapter 35: A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood

Chapter 36: Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry