Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews

ISBN-13: 9781617374289 / Angielski / Miękka / 2010 / 398 str.

Neil Woodford; Alan P. Johnson

Opis

Gazing into crystal balls is beyond the expertise of most scientists. Yet, as we look further into the 21st century, one does not have to be Nostradamus to predict that the current genomics and proteomics "revolution" will have an immense impact on medical bacteriology. This impact is already being re- ized in many academic departments, and although encroachment on routine diagnostic bacteriology, particularly in the hospital setting, is likely to occur at a slower pace, it remains nonetheless inevitable. Therefore, it is important that no one working in bacteriology should find themselves distanced from these fundamental developments. The involvement of all clinical bacteriologists is essential if the significant achievements of genome sequencing and analysis are to be turned into tangible advances, with resulting benefits for patient care and m- agement. It is our hope that Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews will play a part in bringing such a development to fruition. The advances in genomics and proteomics have already given us frequent opportunities to reassess our knowledge and understanding of established b- terial adversaries, and have provided us with the means to identify new foes. The new knowledge gained is enabling us to reconsider, for example, our c- cepts of bacterial pathogenicity, phylogeny and novel targets for antibacterial chemotherapy. These topics, and others, are considered in Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews.

Spis treści

Part I. Principles of Bacterial Genomics Bacterial Genomes for the Masses: Relevance to the Clinical Laboratory Mark J. Pallen Public Databases: Retrieving and Manipulating Sequences for Beginners Neil Woodford Genome Sequencing and Annotation: An Overview Makoto Kuroda and Keiichi Hiramatsu Comparative Genomics: Digging for Data Matthew B. Avison Genome Plasticity: Insertion Sequence Elements, Transposons and Integrons, and DNA Rearrangement Peter M. Bennett Exploring and Exploiting Bacterial Proteomes Stuart J. Cordwell Part II. Application of Genomics to Diagnostic Bacteriology Molecular Diagnostics: Current Opinions B. Cherie Millar and John E. Moore Molecular Diagnostics: Future Probe-Based Strategies Peter Marsh and Donald L. N. Cardy Real-Time PCR Nicholas A. Saunders Microarrays for Bacterial Typing: Realistic Goal or Holy Grail? Carola Van Ijperen and Nicholas A. Saunders Part III. Interrogating Bacterial Genomes Genomic Approaches to Antibacterial Discovery David J. Payne, Michael N. Gwynn, David J. Holmes, and Marty Rosenberg Using the Genome to Understand Pathogenicity Dawn Field, Jennifer Hughes, and E. Richard Moxon Identification of Novel Pathogenicity Genes by PCR Signature-Tagged Mutagenesis and Related Technologies Dario E. Lehoux, François Sanschagrin, Irena Kukavica-Ibrulj, Eric Potvin, and Roger C. Levesque Discovering New Pathogens: Culture-Resistant Bacteria Andrew J. Lawson Exploring the Concept of Clonality in Bacteria Brian G. Spratt Bacterial Taxonomics: Finding the Wood Through the Phylogenetic Trees Robert J. Owen Index

Blurb

It is essential that all clinical bacteriologists become active participants in the current genomics and proteomics revolution if the significant achievements of genome sequencing and analysis are to produce real benefits for patient care. In Genomics, Proteomics, and Clinical Bacteriology, a panel of internationally renowned experts reviews how genomics has provided novel methods for bacterial investigation and advanced our knowledge of bacterial pathogenicity. The authors critically evaluate the applications of genomics to diagnostic bacteriology, highlighting both current and likely future uses, describing real-time PCR methods, and outlining the promise of microarrays in clinical bacteriology. Their discussion examines in detail genomic approaches to antibacterial discovery, the nature of pathogenicity, the discovery of new pathogens, the exploration of the concept of clonality in bacteria, and bacterial taxonomics. Introductory material explains for the uninitiated the relevance of genomics to the clinical laboratory, illustrating the art and science of working with public databases, digging for data, and exploiting bacterial proteomes.
Comprehensive and insightful, Genomics, Proteomics, and Clinical Bacteriology offers everyone working in medical bacteriology an accessible introduction to a rapidly evolving discipline, one that shows not only how knowledge of bacterial genome sequences affects diagnostic bacteriology today, but also how that knowledge may be used in the future to gain new insights into bacterial disease processes, identify critical targets for antiinfectives, and aid in designing novel antibiotics.