Fluorescence Spectroscopy, Imaging and Probes: New Tools in Chemical, Physical and Life Sciences

ISBN-13: 9783642627323 / Angielski / Miękka / 2012 / 390 str.

Ruud Kraayenhof; Antonie J. W. G. Visser; Hans C. Gerritsen

Opis

The application of fluorescence in drug discovery, high-throughout screenings in genomics and proteomics is and will be evidently successful. The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences. This topic book reflects the updates of scientific progress presented by frontline researchers.

Spis treści

Fluorescence Spectroscopy: New Approaches and Probes.- Advanced Luminescent Labels, Probes and Beads and their Application to Luminescence Bioassay and Imaging.Fluorescence Spectral Engineering- Biophysical and Biomedical Applications.Fluorescence Nanometrology in Sol-Gels.Integrated Supramolecular Systems: from Sensors to Switches.Ratiometric Probes: Design and Applications.Binding of Ethidium to Yeast tRNAPhe:A New Perspective on an Old Bromide.Experimental Aspects of DNA Computing by Blocking: Use of Fluorescence Techniques for Detection.- Fluorescence Spectroscopy of Single Molecules and Molecular Assemblies. Multiparametric Detection of Fluorescence Emitted from Individual Multichromophoric Systems.Fluorescence Intensity Distribution Analysis (FIDA) and Related Fluorescence Fluctuation Techniques: Theory and Practice.Single Molecule Reactions of the Enzyme LDH and of Restriction Endonucleases in the Fluorescence Microscope.- Application of Fluorescence in Biological Membrane and Enzyme Studies.Application of the Wavelength-Selective Fluorescence Approach to Monitor Membrane Organization and Dynamics.Fluorescence Approaches for the Characterisation of the Peripheral Membrane Binding of Proteins Applied for the Blood Coagulation Protein Prothrombin.Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-Photon Scanning Fluorescence Microscopy.Formation of Higher Order Signal Transduction Complexes as seen by Fluorescence Spectroscopy.Mechanisms of Modulation of the Modulation of Membrane Interfacial Enzyme Catalysis by Non-lamellar Forming Lipids: Comparison with the Behaviour of a Fluorescent Probe in Membranes.Emission Spectroscopy of Complex Formation between E. coli Purine Nucleoside Phosphorylase (PNP) and Identified Tautomeric Species of Formycin Inhibitors Resolves Ambiguities found in Crystallographic Studies.- Fluorescence Microscopic Imaging Techniques for the Study of Living Cells.Fluorescence Lifetime Imaging Implemented with Resonant Galvanometer Scanners.Spectral Imaging of Single CdSe/ZnS Quantum Dots Employing Spectrally- and Time-Resolved Confocal Microscopy.Imaging of Oxidative Stress in Plant Cells by Quantitative Fluorescence Microscopy and Spectroscopy.The Biomedical Use of Rescaling Procedures in Optical Biopsy and Optical Molecular Imaging.Looking into a living cell.Expression of Multicolor Fluorescent Fusion Proteins in Zebrafish Cell Cultures: A Versatile Tool in Cell Biology

Blurb

Fluorescence techniques enjoy ever-increasing interest from a multitude of disciplines: physics, chemistry, biology, geology, pharmacology, toxicology and medicine. Besides widespread fundamental and applied research of fluorescence in university laboratories, one observes a substantially enhanced effort by smaller and larger companies towards the development of new fluorescence-based diagnostic tools. This increased use of fluorescence techniques is greatly enhanced by the improved instrumentation, in particular that of microscopic imaging, such as confocal scanning and multi-photon excitation microscopies. Moreover, the development of many new molecular probes with higher selectivity for specific micro-environmental properties has stimulated many new researchers to employ fluorescence techniques. This topic book, the second in the Springer Series on Fluorescence, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.