Expression, Purification, and Structural Biology of Membrane Proteins

ISBN-13: 9781071603758 / Angielski / Miękka / 2020 / 423 str.

Camilo Perez; Timm Maier

Spis treści

1. Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae

            Porsha L.R. Shaw, Kathryn A. Diederichs, Ashley Pitt, Sarah E. Rollauer, and Susan K. Buchanan

 

2. Membrane Protein Production in Escherichia coli

            Benjamin C. McIlwain and Ali A. Kermani

 

3. Membrane Protein Production in Lactococcus Lactis for Structural Studies

            Chloe Martens

 

4. Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae

            Martin S. King and Edmund R.S. Kunji

 

5. Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System

            Jacques Boivineau, Matthias Haffke, and Veli-Pekka Jaakola

 

6. Membrane Extracts from Plant Tissues

            Uwe Bodensohn, Christoph Ruland, Roman Ladig, and Enrico Schleiff

 

7. Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter

            Charlott Stock and Inga Hänelt

 

8. GPCR Solubilization and Quality Control

            Tamara Miljus, David A Sykes, Clare Harwood, Ziva Vuckovic, and Dmitry B. Veprintsev

 

9. Affinity Purification of Membrane Proteins

            Elisabeth Graeber and Volodymyr M. Korkhov

 

10. Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage

            Stephan Hirschi and Dimitrios Fotiadis

 

11. Biotinylation of Membrane Proteins for Binder Selections

            Benedikt T. Kuhn, Iwan Zimmermann, Pascal Egloff, Lea M. Hürlimann, Cedric A.J. Hutter, Christian Miscenic, Roger J.P. Dawson, Markus A. Seeger, and Eric R. Geertsma

 

12. Production and Application of Nanobodies for Membrane Protein Structural Biology

            Janine Denise Brunner and Stephan Schenck

 

13. Identifying Conformation-Selective Heavy-Chain-Only Antibodies against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay

            Peter Stohler and Roger J.P. Dawson

 

14. Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses

            Philipp A.M. Schmidpeter, Nattakan Sukomon, and Crina M. Nimigean

 

15. Reconstitution of Proteoliposomes for Phospholipid Scrambling and Non-Selective Channel Assays

            Maria E. Falzone and Alessio Accardi

 

16. Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent

            Magnus Bloch, Mònica Santiveri, and Nicholas M.I. Taylor

 

17. Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs

            Valeria Kalienkova, Carolina Alvadia, Vanessa Clerico Mosina, and Cristina Paulino

 

18. Fast Small-Scale Membrane Protein Purification and Grids Preparation for Single Particle Electron Microscopy

            Natalie Bärland and Camilo Perez

 

19. Stabilization and Crystallization of a Membrane Protein Involved in Lipids Transport

            Bing Zhang and Camilo Perez

 

20. In Meso In Situ Serial X-ray Crystallography (IMISX): A Protocol for Membrane Protein Structure Determination at the Swiss Light Source

            Chia-Ying Huang, Vincent Olieric, Martin Caffrey, and Meitian Wang

 

21. Membrane Protein Preparation for Serial Crystallography Using High Viscosity Injectors: Rhodopsin as an Example

            Tobias Weinert and Valérie Panneels

 

22. Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins

            Darragh P. O’Brien, Véronique Hourdel, Alexandre Chenal, and Sébastien Brier

 

23. Mechanical Unfolding and Refolding of Single Membrane Proteins by Atomic Force Microscopy

            Noah Ritzmann and Johannes Thoma

 

24. Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins

            Hundeep Kaur, Anne Grahl, Jean-Baptiste Hartmann, and Sebastian Hiller

 

25. Exploring Lipid- and Membrane Protein-Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy

            Stefan Bibow

Blurb

This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins.

Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.