Essentials of Medical Genomics

ISBN-13: 9780470140192 / Angielski / Twarda / 2008 / 456 str.

Jr. Stuart M. Brown; John G. Hay; Harry Ostrer

Opis

Six new chapters on vital topics of interest such as multilocus SNP genotyping (SNP chips), RNAi, ChIP-chip, and genomic tiling arrays New edition responds to reviewers and users desire for greater coverage--now the most useful handbook on the market! Practical, concise summary of everything about genomics and emerging technologies a busy physician or medical student should know Covers concepts and techniques that are in use in medicine now, as well as those on the cutting-edge of science relevant to medicine, from bioinformatics to DNA diagnostics and proteomics NEW: Includes chapter-end exercises, enhancing the utility of the new edition as a textbook NEW: PowerPoint slides of images available at instructor website

Spis treści

Preface.

1 Introduction to Molecular Genetics.

The Principles of Inheritance.

Genes Are Made of DNA.

DNA Structure.

The Central Dogma.

References.

2 Molecular Biology Technology.

Cut, Copy, and Paste.

Restriction Enzymes.

DNA Cloning Is Copying.

PCR Is Cloning without the Bacteria.

DNA Sequencing.

References.

3 Genome Databases.

Genome Sequencing.

Entrez.

BLAST.

Genome Annotation.

Genome Browser.

Human Genetic Diseases.

A System for Naming Genes.

Model Organisms (Comparative Genomics).

Sequencing Other Genomes.

References.

4 Bioinformatics Tools.

Patterns and Tools.

Sequence Comparison.

Multiple Alignment.

Pattern Finding.

Phylogenetics.

Biotechnology Exercise.

References.

5 Human Genetic Variation.

Mutation.

Single–Nucleotide Polymorphisms.

Linkage.

Multigene Diseases.

Genetic Testing.

SNP Chips.

The HapMap Project.

Research Uses of SNP Markers.

Ethnicity and Genome Diversity.

References.

6 Genetic Testing for the Practitioner (Harry Ostrer).

Clinical Applications of Genetic Testing.

Methods of Genetic Testing.

Adequacy of Genetic Testing.

Informed Consent.

Genetic Counseling.

Clinical Vignettes.

References.

7 Gene Therapy (John G. Hay).

Historical Perspective.

Strategies of Gene Therapy.

DNA Elements for Gene Expression.

Gene Delivery Systems.

Targeting Gene Delivery.

Formative Years and Initial Clinical Approaches.

The Problems.

The Future.

References.

8 Microarrays.

Spotting versus Synthesis on the Chip.

Other Types of Arrays.

Differential Gene Expression.

Error and Reliability.

Evolutionary Perspectives.

References.

9 Analysis of Microarray Data.

Experimental Design.

Data Analysis Workflow.

Functional Analysis.

Validation.

References.

10 Pharmacogenomics and Toxicogenomics.

Pharmacogenomics.

Environmental Chemicals.

Toxicogenomics for Drug Development.

References.

11 Clinical Research Informatics.

Clinical Databases.

Clinical Trials Management.

Data Standards and Ontologies.

Tissue Banks.

Application to Medical Practice.

References.

12 RNA Interference and MicroRNAs.

Antisense RNA.

RNA Interference.

Ribozymes.

References.

13 Alternative Splicing.

Exon Arrays.

Medical Applications of Alternative Splicing.

References.

14 Genome Tiling Chips.

Genome Chips.

Resequencing Chips.

Whole–Genome Transcription Profilig.

ChIP–chip.

ArrayCGH.

References.

15 Cancer Genomics.

Understanding Cancer Genomics.

Copy Number Mutations.

Gene Expression Signatures.

Cancer Genome Atlas.

References.

16 Proteomics.

Protein Modifications.

Quantitative Approaches.

Biomarkers.

Protein Databases.

Protein Protein Interactions.

DNA–Binding Proteins.

Structural Proteomics.

Drug Targets.

References.

17 Consumer Genomics and Genealogy.

Genealogy.

Nutrigenomics.

Privacy Concerns.

References.

18 The Ethics of Medical Genomics.

Eugenics.

Human Genome Diversity Project and Population Genetics.

Genetic Discrimination.

Impact on Physicians and Researchers.

Clinical Research.

References.

Appendix: Genetic Testing: Scientific Background for Policymakers (Amanda K. Sarata).

Glossary.

Index.

O autorze

STUART M. BROWN, PhD, is on the faculty of the New York University School of Medicine, where he is Associate Professor and Director of the Bioinfor–matics Core Facility and Director of the graduate Bioinformatics course.

Blurb

An up–to–date edition of the groundbreaking classic

Medical genomics is rapidly moving into mainstream medicine, with new and emerging technologies such as molecular genetic diagnostic tests and gene therapy having an unprecedented impact in clinical practice. Consequently, there is an urgent need for physicians and medical students to understand these developments and their evolving roles in the post–genomics era. This book fills that important need with a practical, comprehensive introduction to genomics with a focus on its impact on medical research and practice.

This valuable new edition has been thoroughly and meticulously updated and expanded to include the most exciting and up–to–the–minute topics in biomedical research, including all–new chapters on multilocus SNP genotyping (SNP chips), RNAi, ChIP–chip, and genomic tiling arrays. It also includes thoroughly revised coverage of topics from the previous edition, including molecular biology, biotechnology, genome databases, bioinformatics tools, human genetic variation, genetic testing, gene therapy, microarray and related gene expression technology, analysis of microarray data, pharmacogenomics and toxico–genomics, clinical research informatics, alternative splicing, cancer genomics, proteomics, consumer genomics, and genetic data privacy and ethics.

Covering concepts and techniques that are currently in use, as well as those on the cutting–edge of science, Essentials of Medical Genomics, Second Edition gives physicians and medical students everything they need to know about genomics and emerging technologies. Now 50% more comprehensive than the previous edition, and complemented with useful exercises and an appendix, this Second Edition is truly the most useful handbook available.