Drought related MYB transcription factor gene from Brassica : Isolation, Cloning and Characterization of Drought related MYB transcription factor gene from Brassica carinata

ISBN-13: 9783659936784 / Angielski / Miękka / 2016 / 112 str.

Supriya Ambawat

Opis

There is a growing interest in use of regulatory genes as an effective way of developing stress tolerant genotypes as plants respond by modulating gene expression leading to cellular homeostasis, detoxification and recovery of growth. Identification of novel transcription factor genes and their role in regulating the expression of stress induced genes will help in understanding signaling pathways leading to stress tolerance. Genetic engineering is attractive because of its potential to improve abiotic stress tolerance more rapidly as compared to traditional plant breeding. Myb gene family is represented by 190 genes in Arabidopsis but there are no reports in Brassicas so far. This book contains the first report on isolation and cloning of drought related MYB transcription factor gene from Brassica carinata using RT-PCR approach. Three sets of primers specific to Myb gene were used and the gene was successfully cloned, characterized and confirmed by plasmid DNA isolation, colony PCR, RE digestion. This description will be very helpful as the plant transformation using identified regulatory genes is encouraging and useful for developing improved cultivars for drought tolerance.

O autorze

Dr. Supriya has earned her M.Sc. and Ph.D. (Biotechnology and Molecular Biology) from CCS HAU, Hisar in 2006 and 2010 respectively. She has been a Ph.D. Research Scholar at ICRISAT, Hyderabad from 2008-2010. She has excellent academic and publication record with 30 publications to her credit. Presently she is working in areas such as Biotechnology,