Coconut Oil - the Best Alternative for Xylene in H&E Staining : A comparative experimental study

ISBN-13: 9786200471161 / Angielski

Sarvesh Vijay

Opis

Considering the toxicity of xylene, it is desirable to minimize its use in histopathology laboratory without compromising the staining quality and hence the appropriate diagnosis. The present study concludes that among three different oils, coconut oil was the best alternative for xylene in every aspect. The findings of this study shows that xylene free H and E staining procedure carried out using coconut oil produced slides with sufficient nuclear, cytoplasmic staining, clarity and uniformity of staining. It also has added advantages of being cost effective, non-toxic, non-inflammable, nonhazardous, biodegradable, economic, easy to handle, readily available and maintains cellular architecture of tissue sections.The only drawback associated with coconut oil, is its tendency to get solidified at a lower temperature. This could, however, be overcome by performing the dewaxing procedure in an incubator maintaining the required temperature.

O autorze

Vijay, Sarvesh
Sarvesh Vijay is a dynamic, accomplished and talented dental professional graduated in year 2014. Nevertheless, in the year 2015 his hunger for specialized knowledge in the field led to his choice in Masters of Dental Surgery Program. By then he had taken interest in Research and Innovation and has published various research papers internationally.